Multiple potential germ-line helicases are components of the germ-line-specific P granules of Caenorhabditis elegans
Multiple potential germ-line helicases are components of the germ-line-specific P granules of Caenorhabditis elegans
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DOI：
10.1073/pnas.93.24.13837
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发表时间：
1996-11-26
影响因子：
11.1
通讯作者：
Bennett, KL
中科院分区：
文献类型：
--
作者：
Gruidl, ME;Smith, PA;Bennett, KL
线虫生殖系特异性P颗粒的两种成分已经用针对每种成分的多克隆抗体进行了鉴定。这两种成分都是推测的生殖系RNA解旋酶(GLHS)，含有在逆转录病毒的RNA结合核衣壳蛋白中发现的CCHC锌指。预测的GLH-1蛋白有四个CCHC指；GLH-2有六个。在生殖系发育的所有阶段，这两种GLH蛋白都定位在P颗粒中。然而，这两个glh基因在两性生殖系和雄性生殖系中显示出不同的RNA和蛋白质积累模式。将反义glh-1或glh-2RNA注射到野生型蠕虫中，会导致一些后代发育成不育的成虫，这表明正常的生殖系发育需要其中一个或两个基因。由于这些非常相似的glh基因在物理上相互映射在几百个千碱基以内，它们似乎代表了一种相当新的基因复制事件。
Two components of the germ-line-specific P granules of the nematode Caenorhabditis elegans have been identified using polyclonal antibodies specific for each. Both components are putative germ-line RNA helicases (GLHs) that contain CCHC zinc fingers of the type found in the RNA-binding nucleocapsid proteins of retroviruses. The predicted GLH-1 protein has four CCHC fingers; GLH-2 has six. Both GLH proteins localize in the P granules at all stages of germ-line development. However, the two glh genes display different patterns of RNA and protein accumulation in the germ lines of hermaphrodites and males. Injection of antisense glh-1 or glh-2 RNA into wild-type worms causes some offspring to develop into sterile adults, suggesting that either or both genes are required for normal germ-line development. As these very similar glh genes physically map within several hundred kilobases of one another, it seems likely that they represent a fairly recent gene duplication event.