Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes
Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes
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DOI：
10.1016/j.gene.2005.11.015
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发表时间：
2006-03-29
期刊：
影响因子：
3.5
通讯作者：
Hori, H
中科院分区：
文献类型：
--
作者：
Kurosawa, G;Takamatsu, N;Hori, H
我们分离出BAC克隆，覆盖了青鳉鱼的整个hox基因位点。BAC克隆的特征在于与许多hox基因探针在我们以前的研究中分离的Southern杂交和PCR使用的引物设计用于选择性扩增各自的hox基因。然后，对BAC克隆进行鸟枪测序。结果揭示了整个hox基因位点的组织。共编码46个hox基因，分为7个簇：Aa簇10个，Ab簇5个，Ba簇9个，Bb簇4个，Ca簇10个，Da簇6个，Db簇2个。结合河豚基因组数据库和Danio基因组数据库中的hox基因位点信息，构建了三种鱼类基因组的物理图谱，并进行了相互比较。青鳉和河豚不仅hox基因的数目非常接近，而且相邻hox基因之间的距离也非常接近。Aa、Ab、Ba. Bb、Ca和Da在三种鱼类中普遍存在，在每个聚类中只有很少或没有差异。因此，hox基因集应该已经很好地保存，一旦他们已经建立在各自的物种。(c)2005 Elsevier B. V保留所有权利。
We isolated BAC clones that cover the entire hox gene loci in the medaka fish Oryzias latipes. The BAC clones were characterized by the Southern hybridization with many hox gene probes isolated in our previous study and by PCR using primers designed for selective amplification of respective hox genes. Then, the BAC clones have been subjected to shotgun sequencing. The results revealed the organization of the entire hox gene loci. Forty-six hox genes in total are encoded in seven clusters as follows: 10 hox genes in Aa cluster; 5 in Ab; 9 in Ba; 4 in Bb; 10 in Ca; 6 in Da; and 2 in Db. Together with the information on the hox gene loci registered in the Fugu genome database and in the Danio genome database, the physical maps of three fish genomes were constructed and compared one another. Not only numbers of hox genes but also the distances between the neighboring hox genes are highly similar between medaka and fugu. As for six clusters, Aa, Ab, Ba. Bb, Ca and Da that are commonly present in the three fishes, only few or no differences were found in each cluster. Thus, the hox gene sets should have been well conserved once they had been established in respective species. (c) 2005 Elsevier B.V All rights reserved.