The SK2-long isoform directs synaptic localization and function of SK2-containing channels.
The SK2-long isoform directs synaptic localization and function of SK2-containing channels.
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DOI：
10.1038/nn.2832
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发表时间：
2011-06
影响因子：
25
通讯作者：
Adelman, John P.
中科院分区：
文献类型：
--
作者：
Allen, Duane;Bond, Chris T.;Lujan, Rafael;Ballesteros-Merino, Carmen;Lin, Mike T.;Wang, Kang;Klett, Nathan;Watanabe, Masahiko;Shigemoto, Ryuichi;Stackman, Robert W., Jr.;Maylie, James;Adelman, John P.
含有sk2的通道在小鼠CA1锥体神经元的树突棘突触后密度（PSD）中表达，并影响突触反应、可塑性和学习。SK2基因编码两种异构体，仅在n端结构域的长度上不同。SK2-Long （SK2-L）和SK2-Short （SK2-S）在CA1锥体神经元中共表达，并可能形成异质通道。在缺乏SK2-L的小鼠（仅sk2 - s小鼠）中，含有sk2 - s的通道在突触外膜中表达，但在PSD中被排除。在SK2-Sonly小鼠中，SK通道对epsp的贡献缺失，并通过SK2-L的重新表达得以恢复。在野生型小鼠的切片中，阻断SK通道增加了CA1区诱导的长期增强（LTP）的数量，但对SK2-Sonly小鼠没有影响。此外，SK2-Sonly小鼠在新的目标识别任务中表现优于野生型小鼠。这些结果表明，SK2-L指导突触中含有sk2的通道表达，对正常的突触信号传导、可塑性和学习很重要。
SK2-containing channels are expressed in the postsynaptic density (PSD) of dendritic spines on mouse CA1 pyramidal neurons, and influence synaptic responses, plasticity, and learning. The SK2 gene encodes two isoforms differing only in the length of the N-terminal domain. SK2-Long (SK2-L) and SK2-Short (SK2-S) are co-expressed in CA1 pyramidal neurons and likely form heteromeric channels. In mice lacking SK2-L (SK2-Sonly mice), SK2-S-containing channels were expressed in the extrasynaptic membrane, but were excluded from the PSD. The SK channel contribution to EPSPs was absent in SK2-Sonly mice, and was restored by SK2-L re-expression. In slices from wild type mice, blocking SK channels increased the amount of long-term potentiation (LTP) induced in area CA1 but was without effect in SK2-Sonly mice. Further, SK2-Sonly mice outperformed wild type mice in the novel object recognition task. These results show that SK2-L directs synaptic SK2-containing channel expression, important for normal synaptic signaling, plasticity, and learning.
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影响因子：
25
作者：
Lin, Mike T.;Lujan, Rafael;Maylie, James
通讯作者：
Maylie, James
影响因子：
56.9
作者：
Bond, CT;Sprengel, R;Adelman, JP
通讯作者：
Adelman, JP
影响因子：
2
作者：
Stackman, Robert W., Jr.;Bond, Chris T.;Adelman, John P.
通讯作者：
Adelman, John P.
影响因子：
2.5
作者：
Harris, Alexander Z.;Pettit, Diana L.
通讯作者：
Pettit, Diana L.
影响因子：
16.2
作者：
Bloodgood, Brenda L.;Sabatini, Bernardo L.
通讯作者：
Sabatini, Bernardo L.