Assessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions
Assessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions
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DOI：
10.1016/j.aquatox.2006.02.015
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发表时间：
2006-06-01
影响因子：
4.5
通讯作者：
Sciutto, Andrea
中科院分区：
文献类型：
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作者：
Bolognesi, Claudia;Perrone, Emanuela;Sciutto, Andrea
本研究的目的是标准化和评估鱼类红细胞MN试验细胞遗传学分析作为海洋环境污染生物标志物的预测价值。对参考区域(S.Teresa.)的一些鱼类物种的红细胞中的MN频率基线进行了评估。一些常见的化学污染物和混合物的遗传毒性潜力是在受控条件下在水族馆中实验暴露的鱼中测定的。将大菱形鱼暴露在50 ppb的单一化学物质(二烷基多磺酸)中3周。双酚A、四溴联苯醚)、30 ppb的壬基酚和混合物(北海石油和含烷基酚的北海石油)。染色体损伤以鱼红细胞微核(MN)率为指标。还记录了核异常，如泡状、凹陷和分叶状核。双酚A和四溴联苯醚可显著增加鱼类红细胞微核率。化学混合物北海油+烷基化酚诱导的微核率最高(2.95个微核率/1000个细胞，对照组为1个微核率/1000个细胞)。研究结果表明，微核试验作为累积暴露的指标，在受控条件下可作为评价鱼类遗传毒性化合物的灵敏模型。(C)2006爱思唯尔B.V.保留所有权利。
The aim of the present study was to standardize and to assess the predictive value of the cytogenetic analysis by MN test in fish erythrocytes as a biomarker for marine environmental contamination. MN frequency baseline in erythrocytes was evaluated in a number of fish species from a reference area (S. Teresa. La Spezia Gulf) and genotoxic potential of a number of common chemical contaminants and mixtures was determined in fish experimentally exposed in aquarium under controlled conditions. Fish (Scophthalmus maximus) were exposed for 3 weeks to 50 ppb of single chemicals (dialkyl plithalate. bisphenol A, tetrabromodiphenyl ether), 30ppb nonylphenol and mixtures (North Sea oil and North Sea oil with alkylated phenols). Chromosomal damage was determined as micronuclei (MN) frequency in fish erythrocytes. Nuclear anomalies such as blebbed, notched and lobed nuclei were also recorded. Significant increase in MN frequency was observed in erythrocytes of fish exposed to bisphenol A and tetrabromodiphenylether. Chemical mixture North Sea oil + alkylated phenols induced the highest MN frequency (2.95 micronucleated cells/1000 cells compared to 1 MNcell/1000 cells in control animals). The study results revealed that micronucleus test, as an index of cumulative exposure, appears to be a sensitive model to evaluate genotoxic compounds in fish under controlled conditions. (c) 2006 Elsevier B.V. All rights reserved.