Laser-patterned lateral-flow devices with multiple flow-paths for semi-quantitative measurement of the inflammatory biomarker, C-reactive protein (Conference Presentation)
Laser-patterned lateral-flow devices with multiple flow-paths for semi-quantitative measurement of the inflammatory biomarker, C-reactive protein (Conference Presentation)
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具有多个流路的激光图案侧流装置，用于半定量测量炎症生物标志物 C 反应蛋白（会议演示）
DOI：
10.1117/12.2542692
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Iles A
中科院分区：
文献类型：
--
作者：
Iles A
我们报道了一种新的解决方案，用于半定量检测C反应蛋白(C-反应蛋白)，一种常见的炎症标志物，使用多路径横向流动装置(LFD)，通过使用激光直写技术精确划分标准LFD的单一流动路径而制成。多个隔离的流动路径允许通过单独设置不同的截止水平来检测不同的浓度。通过简单地出现/不存在测试线来观察半定量读数，而不需要阅读器或进一步的分析。我们已经成功地展示了这些多路径LFD用于半定量检测CRP，并将在人体样本中进行验证。
We report a novel solution for semi-quantitative detection of C-reactive protein (CRP), a common inflammatory marker, using a multi-path lateral-flow device (LFD) fabricated via the precise partitioning of the single flow-path of a standard LFD using a laser direct-write technique. The multiple isolated flow-paths allow detection of different concentrations by individually setting different cut-off levels. The semi-quantitative readout is observed via simple appearance/absence of test lines without the need for a reader or further analyses. We have successfully demonstrated the use of these multi-path LFDs for semi-quantitative detection of CRP and will present validation in human samples.