Molecular mapping in oil radish (Raphanus sativus L.) and QTL analysis of resistance against beet cyst nematode (Heterodera schachtii)
Molecular mapping in oil radish (Raphanus sativus L.) and QTL analysis of resistance against beet cyst nematode (Heterodera schachtii)
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DOI：
10.1007/s00122-008-0937-6
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发表时间：
2009-02-01
影响因子：
5.4
通讯作者：
Li, Jinbin
中科院分区：
文献类型：
--
作者：
Budahn, Holger;Peterka, Herbert;Li, Jinbin
甜菜包囊线虫（Heterodera schachtii Schmidt）在甜菜轮作的高感染土壤中可采用抗油萝卜品种进行生物防治。油橄榄属（oleiferus DC.）)作为绿色作物。抗性植物刺激侵染幼体侵入根系，但在其侵入后阻止其完成生命周期。通过添加完整的萝卜染色体，成功地将该抗性性状转移到易感油菜上。本研究的目的是建立萝卜的遗传图谱和开发抗性相关标记。该图谱包含545个RAPD、dpRAPD、AFLP和SSR标记，全长1517 cM，平均距离2.8 cM，包含9个连锁群，大小在120 ~ 232 cM之间。将先前从油菜-萝卜附加系中获得的抗病染色体d和其他8条萝卜染色体的染色体特异性标记封闭为锚点标记。每一个额外的染色体在附加的行可以明确地分配到一个萝卜连锁群。对Pegletta（耐线虫）与Siletta Nova（敏感）杂交的种内F-2定位群体进行了线虫抗性的QTL分析。在染色体d的近端位置检测到一个显性主QTL Hs1 (Rph)，解释46.4%的表型变异。通过染色体附加系，我们获得了已知位置的萝卜染色体特异性锚点标记，用于未来的重组实验，将萝卜中携带所需基因的Hs1 （Rph）片段整合到油菜籽中。
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F-2 mapping population derived from a cross between varieties 'Pegletta' (nematode resistant) x 'Siletta Nova' (susceptible). A dominant major QTL Hs1 (Rph) explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments to incorporate segments carrying desired genes as Hs1 (Rph) from radish into rapeseed by means of chromosome addition lines.