Molecular characterisation of non-absorptive and absorptive enterocytes in human small intestine
Molecular characterisation of non-absorptive and absorptive enterocytes in human small intestine
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DOI：
10.1136/gut.2005.073262
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发表时间：
2006-08-01
期刊：
影响因子：
24.5
通讯作者：
Gretz, N.
中科院分区：
文献类型：
--
作者：
Gassler, N.;Newrzella, D.;Gretz, N.
背景和目标：人类小肠的隐窝-绒毛轴分化的扰动与几种具有临床重要性的肠道疾病相关。目前，小肠上皮细胞在隐窝-绒毛轴的分化还没有很好的characteristic.Subjects和方法：表达谱的微切割肠上皮细胞内衬的上部隐窝或中间的绒毛进行了使用的Affytek X3 P阵列和几种方法进行确认。结果：共发现978个差异表达序列，代表778个独特的UniGene ID，并分为四个功能组。在肠腺上部的肠上皮细胞中，细胞周期促进基因和转录/翻译相关基因的表达占优势，而在绒毛中部的肠上皮细胞中，细胞周期抑制基因、代谢相关基因和囊泡/转运相关基因的表达较高。在分子水平上观察到两种类型的肠上皮细胞，非吸收性肠上皮细胞位于肠腺上部，吸收性肠上皮细胞位于绒毛中部。这些数据提高了我们对人类小肠中隐窝-绒毛结构的生理学知识，并为病理生理学现象提供了新的见解，如临床上重要的绒毛萎缩。
Background and aims: Perturbation of differentiation of the crypt-villus axis of the human small intestine is associated with several intestinal disorders of clinical importance. At present, differentiation of small intestinal enterocytes in the crypt-villus axis is not well characterised.Subjects and methods: Expression profiling of microdissected enterocytes lining the upper part of crypts or the middle of villi was performed using the Affymetrix X3P arrays and several methods for confirmation.Results: A total of 978 differentially expressed sequences representing 778 unique UniGene IDs were found and categorised into four functional groups. In enterocytes lining the upper part of crypts, cell cycle promoting genes and transcription/translation related genes were predominantly expressed, whereas in enterocytes lining the middle of villi, high expression of cell cycle inhibiting genes, metabolism related genes, and vesicle/transport related genes was found.Conclusion: Two types of enterocytes were dissected at the molecular level, the non-absorptive enterocyte located in the upper part of crypts and the absorptive enterocyte found in the middle of villi. These data improve our knowledge about the physiology of the crypt-villus architecture in human small intestine and provide new insights into pathophysiological phenomena, such as villus atrophy, which is clinically important.