Cellular responses to ionizing radiation change quickly over time during early development in zebrafish
Cellular responses to ionizing radiation change quickly over time during early development in zebrafish
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DOI：
10.1002/cbin.11117
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发表时间：
2019-05-01
影响因子：
3.9
通讯作者：
Ichinohe, Tatsuo
中科院分区：
文献类型：
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作者：
Honjo, Yasuko;Ichinohe, Tatsuo
动物细胞不断接收有关环境因素的信息并对其做出反应，包括电离辐射。虽然细胞修复辐射引起的DNA损伤以确保存活至关重要，但胚胎发育早期细胞对辐射的反应仍不清楚。在这项研究中，我们分析了电离辐射对斑马鱼胚胎的影响，发现辐射诱导的γ H2 AX病灶形成和细胞周期停滞直到原肠胚阶段才发生，尽管存在主要的DNA修复相关基因转录本，作为母体因素传递。有趣的是，P21/WAF 1积累开始类似于受精后6小时，虽然p21 mRNA的上调，在受精后2或4小时的辐射。这些结果表明，斑马鱼胚胎在受精后2或4小时的细胞反应，以辐射未能克服P21蛋白的积累和进一步的信号。P21/WAF 1蛋白稳定性的调节似乎是早期胚胎发生过程中对遗传毒素反应的关键因素。
Animal cells constantly receive information about and respond to environmental factors, including ionizing radiation. Although it is crucial for a cell to repair radiation-induced DNA damage to ensure survival, cellular responses to radiation exposure during early embryonic development remain unclear. In this study, we analyzed the effects of ionizing radiation in zebrafish embryos and found that radiation-induced gamma H2AX foci formation and cell cycle arrest did not occur until the gastrula stage, despite the presence of major DNA repair-related gene transcripts, passed on as maternal factors. Interestingly, P21/WAF1 accumulation began similar to 6 h post-fertilization, although p21 mRNA was upregulated by irradiation at 2 or 4 h post-fertilization. These results suggest that the cellular responses of zebrafish embryos at 2 or 4 h post-fertilization to radiation failed to overcome P21 protein accumulation and further signaling. Regulation of P21/WAF1 protein stabilization appears to be a key factor in the response to genotoxins during early embryogenesis.