ALTERNATIVE MECHANISMS OF CAK ASSEMBLY REQUIRE AN ASSEMBLY FACTOR OR AN ACTIVATING KINASE
ALTERNATIVE MECHANISMS OF CAK ASSEMBLY REQUIRE AN ASSEMBLY FACTOR OR AN ACTIVATING KINASE
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DOI：
10.1016/0092-8674(95)90233-3
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发表时间：
1995-10-06
期刊：
影响因子：
64.5
通讯作者：
MORGAN, DO
中科院分区：
文献类型：
--
作者：
FISHER, RP;JIN, P;MORGAN, DO
我们克隆了编码 p36 的小鼠 cDNA，p36 是 CDK 激活激酶 (CAK) 的一个新亚基。 p36 含有 C3HC4 锌结合结构域或环指，并与 CAK 的 TFIIH 结合形式和游离三聚体形式相关。 p36 在体外促进 CDK7 和细胞周期蛋白 H 的组装，稳定瞬时 CDK7-细胞周期蛋白 H 复合物。 p36 对 CAK 的稳定和激活与 T170（CDK7 的保守激活残基）的磷酸化状态无关。活性 CDK7-细胞周期蛋白 H 二聚体的组装也可以通过另一种独立于 p36 的途径进行，该途径需要 CAK 激活激酶 (CAKAK) 对 T170 进行磷酸化。因此，CDK7-细胞周期蛋白H复合物的形成可以通过多种机制来实现。
We have cloned a mouse cDNA that encodes p36, a novel subunit of the CDK-activating kinase (CAK). p36 contains a C3HC4 zinc-binding domain or RING finger and is associated both with a TFIIH-bound form of CAK and with a free trimeric form. p36 promotes the assembly of CDK7 and cyclin H in vitro, stabilizing the transient CDK7-cyclin H complex. Stabilization and activation of CAK by p36 is independent of the phosphorylation state of T170, the conserved activating residue of CDK7. Assembly of active CDK7-cyclin H dimers can also occur through an alternative p36-independent pathway that requires phosphorylation of T170 by a CAK-activating kinase, or CAKAK. Thus, CDK7-cyclin H complex formation can be achieved by multiple mechanisms.