Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome.
Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome.
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DOI：
10.1101/pdb.prot080978
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发表时间：
2017-03
影响因子：
--
通讯作者：
Yizhi Cai;Junbiao Dai
中科院分区：
文献类型：
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作者：
Yizhi Cai;Junbiao Dai
从头合成DNA是合成生物学的关键使能技术之一。特别是大规模DNA合成的方法，已经改变了生命科学研究的许多方面，通过设计新的合成生物系统，支持生物学的新发现。本协议详细描述了目前用于合成和组装大片段DNA用于合成酵母基因组计划的方法。该协议包括构建块合成和块组装的说明，每一个都可以作为一个独立的程序来生成感兴趣的合成DNA。
De novo DNA synthesis is one of the key enabling technologies for synthetic biology. Methods for large-scale DNA synthesis, in particular, have transformed many facets of life science research, supporting new discoveries in biology through the design of novel synthetic biological systems. This protocol describes in detail the methods currently being used to synthesize and assemble large pieces of DNA for the synthetic yeast genome project. The protocol includes instructions for building block synthesis as well as chunk assembly, each of which can be used as a stand-alone procedure to generate a synthetic DNA of interest.