Quantification of Immunoglobulin G in Bovine and Caprine Milk Using a Surface Plasmon Resonance-Based Immunosensor
Quantification of Immunoglobulin G in Bovine and Caprine Milk Using a Surface Plasmon Resonance-Based Immunosensor
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DOI：
10.1021/jf903925k
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发表时间：
2010-03-24
影响因子：
6.1
通讯作者：
Rossi, Claire
中科院分区：
文献类型：
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作者：
Crosson, Cyril;Thomas, Daniel;Rossi, Claire
检测牛和羊乳中的初乳对乳制品行业避免负面的经济和技术后果至关重要。免疫球蛋白G （IgG）是初乳存在的最好标志之一。基于表面等离子体共振（SPR）光谱法实时免疫检测牛乳或羊乳中IgG，对两种方法进行了定量评价。通过从传感器图中提取亲和数据来建立校准曲线，或者使用关联和解离阶段后的剩余结合IgG水平，或者使用关联阶段的IgG结合率。结合率法可大大缩短分析时间，分析时间在4 min以下，与小反刍动物的挤奶时间相适应。结合率法比残留结合法检出限更低，精密度和准确度更高，具有更好的分析性能。
Detection of colostrum in bovine and caprine milks is essential for dairy industries to avoid negative economical and technological consequences. One of the best markers of the presence of colostrum is immunoglobulin G (IgG). Two quantification methods have been evaluated for IgG in bovine or caprine milk, based on the real-time immunodetection of IgG by surface plasmon resonance (SPR) spectroscopy. Calibration curves were established by extracting affinity data from the sensorgrams, either using the residual bound IgG level after the association and dissociation phases or using the IgG binding rate during the association phase. The binding rate method allows for substantially reduced analysis times of below 4 min, which make it compatible with the milking time of small ruminants. Moreover, the binding rate method showed a better analytical performance, with lower detection limit and higher precision and accuracy than the residual binding method.