Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus N and NS proteins
Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus N and NS proteins
复制标题
编码水泡性口炎病毒 N 和 NS 蛋白的 mRNA 的核苷酸序列
作者：
C. Gallione;J. Greene;L. Iverson;J. Rose
水疱性口炎病毒(VSV)m RNA编码N蛋白和NS蛋白的全核苷酸序列已从克隆的序列中确定。N蛋白的编码基因全长1,326个核苷酸，不包括多聚腺苷酸。它包含一个用于翻译的开放阅读框架，该框架从5‘端的Aug密码子延伸到编码一个422个氨基酸的蛋白质。已知N和mRNA在5‘-近端的AUG密码子上包含一个主要的核糖体结合位点和另外两个次要的核糖体结合位点。这些次级位点明确地位于N-mRNA序列中的第二和第三密码子上。在这些位点的翻译启动，如果确实发生了，将导致在第二个阅读框架中合成两个小蛋白。编码NS蛋白的VSV和mRNA全长815个核苷酸，不含多聚腺苷，编码222个氨基酸的蛋白质。预测的NS蛋白(25,110)的相对分子质量大约是NS蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶上迁移率预测的一半。缺乏十二烷基硫酸钠与NS蛋白中一个大的负电荷域的结合可以解释这种异常的电泳迁移率。
The complete nucleotide sequences of the vesicular stomatitis virus (VSV) mRNA's encoding the N and NS proteins have been determined from the sequences of cDNA clones. The mRNA encoding the N protein is 1,326 nucleotides long, excluding polyadenylic acid. It contains an open reading frame for translation which extends from the 5'-proximal AUG codon to encode a protein of 422 amino acids. The N and mRNA is known to contain a major ribosome binding site at the 5'-proximal AUG codon and two other minor ribosome binding sites. These secondary sites have been located unambiguously at the second and third AUG codons in the N mRNA sequence. Translational initiation at these sites, if it in fact occurs, would result in synthesis of two small proteins in a second reading frame. The VSV and mrna encoding the NS protein is 815 nucleotides long, excluding polyadenylic acid, and encodes a protein of 222 amino acids. The predicted molecular weight of the NS protein (25,110) is approximately one-half of that predicted from the mobility of NS protein on sodium dodecyl sulfate-polyacrylamide gels. Deficiency of sodium dodecyl sulfate binding to a large negatively charged domain in the NS protein could explain this anomalous electrophoretic mobility.