The Search for a Lipid Trigger: The Effect of Salt Stress on the Lipid Profile of the Model Microalgal Species Chlamydomonas reinhardtii for Biofuels Production.
The Search for a Lipid Trigger: The Effect of Salt Stress on the Lipid Profile of the Model Microalgal Species Chlamydomonas reinhardtii for Biofuels Production.
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DOI：
10.2174/2211550105666160322234434
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发表时间：
2016-11
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影响因子：
--
通讯作者：
Wright PC
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文献类型：
--
作者：
Hounslow E;Kapoore RV;Vaidyanathan S;Gilmour DJ;Wright PC
藻类细胞在受到压力时会产生中性脂肪，这可以用来生产生物柴油。对模拟微藻--莱茵衣藻在盐胁迫下生产生物柴油脂肪的适宜性进行了测试。对无淀粉突变株CC-4325进行了盐胁迫(0.1、0.2和0.3M)处理，通过酯交换和GC分析测定了脂肪酸甲酯(FAME)的含量和分布。研究发现，在盐胁迫条件下，藻类的脂肪酸组成发生了变化，0.1M的氯化钠是藻类可以耐受的，而高浓度的氯化钠(0.2和0.3M)则会导致细胞死亡。在盐条件下，无论是长期暴露在0.1M的氯化钠中，还是短期暴露在0.2M和0.3M的氯化钠中，脂肪含量都会增加。棕榈酸(C16：0)和亚麻酸(C18：3N3)在盐度较高时显著增加。盐分的增加可作为莱茵梭菌的脂质触发剂。
Algal cells produce neutral lipid when stressed and this can be used to generate biodiesel. Salt stressed cells of the model microalgal species Chlamydomonas reinhardtii were tested for their suitability to produce lipid for biodiesel. The starchless mutant of C. reinhardtii (CC-4325) was subjected to salt stress (0.1, 0.2 and 0.3 M NaCl) and transesterification and GC analysis were used to determine fatty acid methyl ester (FAME) content and profile. Fatty acid profile was found to vary under salt stress conditions, with a clear distinction between 0.1 M NaCl, which the algae could tolerate, and the higher levels of NaCl (0.2 and 0.3 M), which caused cell death. Lipid content was increased under salt conditions, either through long-term exposure to 0.1 M NaCl, or short-term exposure to 0.2 and 0.3 M NaCl. Palmitic acid (C16:0) and linolenic acid (C18:3n3) were found to increase significantly at the higher salinities. Salt increase can act as a lipid trigger for C. reinhardtii.