CORTICOTROPIN-RELEASING FACTOR IMMUNOREACTIVITY IN MONKEY NEOCORTEX - AN IMMUNOHISTOCHEMICAL ANALYSIS
CORTICOTROPIN-RELEASING FACTOR IMMUNOREACTIVITY IN MONKEY NEOCORTEX - AN IMMUNOHISTOCHEMICAL ANALYSIS
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DOI：
10.1002/cne.902900412
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发表时间：
1989-12-22
影响因子：
2.5
通讯作者：
CHA, CI
中科院分区：
文献类型：
--
作者：
LEWIS, DA;FOOTE, SL;CHA, CI
促肾上腺皮质激素释放因子（CRF）与某些影响新皮质功能的人类神经精神疾病的病理生理学有关。然而，先前尚未描述过扩展且高度分化的灵长类新皮质中包含 CRF 的结构的解剖学组织。在这项研究中，对新世界松鼠猴 (Saimiri sciureus) 新皮质中 CRF 免疫反应性神经元和过程的分布进行了表征。 CRF 免疫反应性神经元的密度、层状分布和形态外观存在显着的区域差异。最大密度的标记神经元存在于前扣带皮层。在前额叶、顶叶和颞叶皮质的关联区域中，存在明显的 CRF 免疫反应性神经元的各种中间密度。在初级视觉皮层和初级运动皮质中观察到 CRF 免疫反应性神经元数量最少。例如，前扣带皮层中标记神经元的密度几乎是中央前皮层中的五倍。 CRF 免疫反应性神经元也以至少四种不同的层状模式分布。例如，在无颗粒的前扣带皮层中，标记的细胞体分布在整个 II、III 和 V 层。在其他区域，例如后扣带皮层，标记的神经元存在于 II、III 和 IV 层中。相比之下，标记的神经元主要存在于视觉皮层的第二层和浅层第三层，而在颞下皮层，它们主要存在于第四层。 CRF 免疫反应性神经元的两种主要形态类型（由细胞体形状定义）的相对分布也存在区域和层状差异。垂直方向的椭圆形神经元通常具有从每个体极产生的单个树突，最常见于第三层。相比之下，第二层和第四层中标记的神经元往往具有圆形或三角形的胞体。在一些联合皮层的第四层，这些多极神经元与高密度的红色样结构相关，这些红色样结构由垂直阵列中聚集在一起的大型免疫反应性静脉曲张组成。这些结构经常被发现位于锥体神经元胞体的正下方。这些发现与高尔基体浸渍研究的比较强烈表明，CRF 存在于吊灯神经元子集的体细胞和轴突盒中。含有CRF的神经元的异质分布和形态多样性表明CRF可能在猴新皮质的不同区域和层中介导不同的功能。
Corticotropin-releasing factor (CRF) has been implicated in the pathophysiology of certain human neuropsychiatric disorders that affect neocortical function. However, the anatomical organization of CRF-containing structures in the expanded and highly differentiated primate neocortex has not been previously described. In this study, the distribution of CRF-immunoreactive neurons and processes was characterized in the neocortex of New World squirrel monkeys (Saimiri sciureus). Substantial regional differences were present in the density, laminar distribution, and morphological appearance of CRF-immunoreactive neurons. The greatest density of labeled neurons was present in anterior cingulate cortex. A wide range of intermediate densities of CRF-immunoreactive neurons was evident in the association regions of the prefrontal, parietal, and temporal cortices. The lowest numbers of CRF-immunoreactive neurons were observed in the primary visual and primary motor cortices. For example, the density of labeled neurons was nearly five times greater in the anterior cingulate cortex than in the precentral cortex. CRF-immunoreactive neurons were also distributed in at least four different laminar patterns. For example, in the agranular anterior cingulate cortex, labeled cell bodies were distributed throughout layers II, III and V. In other regions, such as the posterior cingulate cortex, labeled neurons were present in layers II, III and IV. In contrast, labeled neurons were predominantly present in layers II and superficial III of the visual cortex, whereas in the inferior temporal cortex, they were present predominantly in layer IV. Regional and laminar differences were also present in the relative distributions of the two major morphological types (as defined by cell body shape) of CRF-immunoreactive neurons. Vertically oriented oval neurons, which frequently had a single dendritic process arising from each somal pole, were most frequently found in layer III. In contrast, the labeled neurons in layers II and IV tended to have a round- or triangular-shaped soma. In layer IV of some association cortices, these multipolar neurons were associated with a high density of red-like structures composed of large immunoreactive varicosities clustered together in vertical arrays. These structures were frequently found to be located immediately below the soma of pyramidal neurons. Comparison of these findings with Golgi impregnation studies strongly suggests that CRF is present in the soma and axonal cartridges of a subset of chandelier neurons. The heterogeneous distribution and morphological diversity of CRF-containing neurons suggest that CRF may mediate distinct functions in different regions and layers of monkey neocortex.