Mechanisms of human neutrophil oxidant production after severe injury.
Mechanisms of human neutrophil oxidant production after severe injury.
复制标题
严重损伤后人中性粒细胞氧化剂产生的机制。
DOI：
10.1067/msy.2001.116923
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发表时间：
2001
期刊：
影响因子：
3.8
通讯作者：
Solomkin,JS
中科院分区：
文献类型：
--
作者：
Quaid,G;Cave,C;Williams,MA;Hennigan,RF;Bokoch,G;Solomkin,JS
BackgroundThe目的，这项研究是为了确定机制，增强氧化剂的生产后，严重injuries.MethodsNeutrophils收获入院24小时内谁有一个损伤的严重程度评分大于16的患者。在N-甲酰-甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）刺激后测量非粘附和粘附中性粒细胞氧化剂的产生。细胞色素b558和胞质组分p47 phox和p67 phox的易位分别通过氧化还原光谱和免疫印迹法测定。流式细胞术测量整合素表达。共聚焦显微镜检查整合素和p47 phox共定位。4名女性和14名男性遭受钝性损伤，平均损伤严重程度评分为22分（范围，16至34分）。非粘附性患者中性粒细胞显示fMLP刺激的氧化剂产生减少，而粘附性中性粒细胞氧化剂产生在溶媒对照组和fMLP刺激组中均增加。烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶组分p47 phox和细胞色素b558被动员到质膜，而p67 phox显示最小的变化。整合素CD 11b a链表达显著增加。共聚焦显微镜显示共定位的p47 phox和一个链CD 11b的患者neutrophils.ConclusionsColocalization的NADPH氧化酶组分和整合素的质膜上可能会调节增强的氧化剂生产在人中性粒细胞严重损伤后。（Surgery 2001;130：669-76.）
BackgroundThe purpose of this study was to determine the mechanisms of enhanced oxidant production after severe injury.MethodsNeutrophils were harvested from patients within 24 hours of admission who had an injury severity score greater than 16. Nonadherent and adherent neutrophil oxidant production was measured after N-formyl-methionyl-leucyl-phenylalanine (fMLP) stimulation. Translocation of cytochrome b558and cytosolic components p47phoxand p67phoxwere determined by oxidation-reduction spectroscopy and immunoblotting, respectively. Flow cytometry measured integrin expression. Integrin and p47phoxcolocalization was examined by confocal microscopy.ResultsEighteen patients were studied within 15 ± 1.4 hours. Four women and 14 men suffered a blunt injury and had a mean injury severity score of 22 (range, 16 to 34). Nonadherent patient neutrophils showed a decrease in fMLP-stimulated oxidant production, whereas adherent neutrophil oxidant production was increased in both the vehicle control and fMLP-stimulated groups. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase components p47phoxand cytochrome b558were mobilized to the plasma membrane, whereas p67phoxshowed minimal change. Integrin CD11b a chain showed a significant increase in expression. Confocal microscopy showed colocalization of p47phoxand a chain CD11b on the plasma membrane of patient neutrophils.ConclusionsColocalization of NADPH oxidase components and integrins may regulate the enhanced oxidant production in human neutrophils after severe injury. (Surgery 2001;130:669-76.)