Characterization of Xenorhabdus isolates from La Rioja ( Northern Spain) and virulence with and without their symbiotic entomopathogenic nematodes (Nematoda: Steinernematidae)
Characterization of Xenorhabdus isolates from La Rioja ( Northern Spain) and virulence with and without their symbiotic entomopathogenic nematodes (Nematoda: Steinernematidae)
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DOI：
10.1016/j.jip.2009.08.007
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发表时间：
2009-10-01
影响因子：
3.4
通讯作者：
Boemare, N. E.
中科院分区：
文献类型：
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作者：
Campos-Herrera, R.;Tailliez, P.;Boemare, N. E.
18个与西班牙昆虫病原线虫属斯氏线虫的Xabridus菌株的特点是使用多相的方法，包括表型和分子方法。两个分离物被归类为Xellichabdus嗜黄杆菌，并与斯氏线虫carpocapsae。16个分离物被归类为博氏短杆菌，其中15个与斯氏线虫相关，1个与斯氏线虫相关。两个X还分离到了bovienii II相，其中一个不稳定相是从S. feltiae菌株Rioja和S. Feltiae菌株BZ.选择4个有代表性的细菌菌株，研究其对斜纹夜蛾（Spodoptera littoralis）的致病性。这4株细菌对S. littoralis分离株在注射后24 h发生败血症，36 h杀死约90%的幼虫，除S.克劳塞伊注射48小时后，后一种分离株在幼虫血淋巴中显示出较低的最终种群（10（7）而不是10（8）CFU/幼虫）和较低的幼虫死亡率（70%而不是95-100%）。线虫-细菌复合体对S.结果表明，除海蜇外，海蜇的幼虫在感染后第5天死亡率达80-90%。kraussei，尽管该菌株在感染后7天达到相似的幼虫死亡率。(C)2009 Elsevier Inc. All rights reserved.
Eighteen Xenorhabdus isolates associated with Spanish entomopathogenic nematodes of the genus Steinernema were characterized using a polyphasic approach including phenotypic and molecular methods. Two isolates were classified as Xenorhabdus nematophila and were associated with Steinernema carpocapsae. Sixteen isolates were classified as Xenorhabdus bovienii, of which fifteen were associated with Steinernema feltiae and one with Steinernema kraussei. Two X. bovienii Phase II were also isolated, one instable phase isolated from S. feltiae strain Rioja and one stable phase from S. feltiae strain BZ. Four representative bacterial isolates were chosen to study their pathogenicity against Spodoptera littoralis with and without the presence of their nematode host. The four bacterial isolates were pathogenic for S. littoralis leading to septicemia 24 h post-injection and killing around 90% of the insect larvae 36 h post-injection, except for that isolated from S. kraussei. After 48 h of injection, this latter isolate showed a lower final population in the larval hemolymph (10(7) instead of 10(8) CFU per larvae) and a lower larval mortality (70% instead of 95-100%). The virulence of the nematode-bacteria complexes against S. littoralis showed similar traits with a significant insect larvae mortality (80-90%) 5 days post-infection except for S. kraussei, although this strain reached similar of larval mortality at 7 days after infection. (C) 2009 Elsevier Inc. All rights reserved.