The fern Adiantum capillus-veneris lacks stomatal responses to blue light
The fern Adiantum capillus-veneris lacks stomatal responses to blue light
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DOI：
10.1093/pcp/pcj048
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发表时间：
2006-06-01
影响因子：
4.9
通讯作者：
Shimazaki, Ken-ichiro
中科院分区：
文献类型：
--
作者：
Doi, Michio;Wada, Masamitsu;Shimazaki, Ken-ichiro
本文研究了细孢子菌属的典型种--蕨类植物铁线蕨气孔对光的响应。孢子体完整叶片的气孔对红光的响应是打开的，但当蓝光叠加在红光上时，气孔不打开。这一结果在分离的铁线蕨表皮中得到了证实。红光诱导的气孔反应不受该蕨类植物中光敏色素和光敏素的嵌合蛋白Phy3突变的影响。在另外三种细孢子虫的蕨类植物中，也观察到缺乏蓝光特有的气孔反应，这三种蕨类植物分别是：凤尾蕨属、蜈蚣亚属和耳状细鳞蕨属。质膜H~+-ATPase激活剂复苏霉素诱导铁线蕨气孔开放和H~+释放。Adiantum Phototroin基因AcPHOT1和AcPHOT2在蕨类保卫细胞中表达。利用AcPHOT1对失去蓝光专一性气孔开放的拟南芥Pho1-Pho2双突变体进行转化，恢复了气孔对蓝光的反应。综上所述，这些结果表明，细孢子蕨属的蕨类植物缺乏蓝光特有的气孔反应，尽管该物种中存在功能性的趋光素和质膜H+-ATPase。
We investigated the responses of stomata to light in the fern Adiantum capillus-veneris, a typical species of Leptosporangiopsida. Stomata in the intact leaves of the sporophytes opened in response to red light, but they did not open when blue light was superimposed on the red light. The results were confirmed in the isolated Adiantum epidermis. The red light-induced stomatal response was not affected by the mutation of phy3, a chimeric protein of phytochrome and phototropin in this fern. The lack of a blue light-specific stomatal response was observed in three other fern species of Leptosporangiopsida, i.e. Pteris cretica, Asplenium scolopendrium and Nephrolepis auriculata. Fusicoccin, an activator of the plasma membrane H+-ATPase, induced both stomatal opening and H+ release in the Adiantum epidermis. Adiantum phototropin genes AcPHOT1 and AcPHOT2 were expressed in the fern guard cells. The transformation of an Arabidopsis phot1 phot2 double mutant, which lost blue light-specific stomatal opening, with AcPHOT1 restored the stomatal response to blue light. Taken together, these results suggest that ferns of Leptosporangiopsida lack a blue light-specific stomatal response, although the functional phototropin and plasma membrane H+-ATPase are present in this species.