Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis
Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis
复制标题
DOI：
10.1111/j.1432-0436.2006.00150.x
复制
发表时间：
2007-06-01
期刊：
影响因子：
2.9
通讯作者：
Thesleff, Irma
中科院分区：
文献类型：
--
作者：
Yamashiro, Takashi;Zheng, Li;Thesleff, Irma
我们探讨了Wnt信号在小鼠磨牙牙本质形成中的作用。我们发现Wnt 10a与成牙本质细胞的分化密切相关，并且在分泌型成牙本质细胞中，Wnt 10a与牙本质涎磷蛋白（Dspp）的表达存在显著的共定位。Dspp是一种牙齿特异性非胶原基质蛋白，调节牙本质矿化。Wnt 10在C3 H10 T1/2细胞中的瞬时过表达诱导了Dspp mRNA。有趣的是，这种诱导只发生在转染细胞培养基质胶基底膜提取物。这些发现表明Wnt 10a是Dspp表达的上游调控分子，并且细胞-基质相互作用对于诱导Dspp表达是必不可少的。此外，Wnt 10a特异性表达于调节牙齿发育的上皮信号中心，初级和次级釉质结。Wnt 10a mRNA的时空分布表明，表达转移从次级釉质结，在未来的尖点的潜在preodontoblasts。表达模式和过表达研究共同表明Wnt 10a是牙本质形成的关键分子，并且它与调节成牙本质细胞分化的细胞-基质相互作用相关。我们的结论是，Wnt 10a可能链接成牙本质细胞的分化和牙尖形态发生。
We have explored the role of Wnt signaling in dentinogenesis of mouse molar teeth. We found that Wnt10a was specifically associated with the differentiation of odontoblasts and that it showed striking colocalization with dentin sialophosphoprotein (Dspp) expression in secretory odontoblasts. Dspp is a tooth specific non-collagenous matrix protein and regulates dentin mineralization. Transient overexpression of Wnt10 in C3H10T1/2, a pluripotent fibroblast cell line induced Dspp mRNA. Interestingly, this induction occurred only when transfected cells were cultured on Matrigel basement membrane extracts. These findings indicated that Wnt10a is an upstream regulatory molecule for Dspp expression, and that cell-matrix interaction is essential for induction of Dspp expression. Furthermore, Wnt10a was specifically expressed in the epithelial signaling centers regulating tooth development, the primary and secondary enamel knots. The spatial and temporal distribution of Wnt10a mRNA demonstrated that the expression shifts from the secondary enamel knots, to the underlying preodontoblasts in the tips of future cusps. The expression patterns and overexpression studies together indicate that Wnt10a is a key molecule for dentinogenesis and that it is associated with the cell-matrix interactions regulating odontoblast differentiation. We conclude that Wnt10a may link the differentiation of odontoblasts and cusp morphogenesis.