Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives.
Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives.
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DOI：
10.7554/elife.66078
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发表时间：
2021-08-16
期刊：
影响因子：
7.7
通讯作者：
Artinger KB
中科院分区：
文献类型：
--
作者：
Lencer E;Prekeris R;Artinger KB
神经嵴是干细胞样细胞的迁移群体，有助于多种特征，包括头骨，周围神经系统和色素。神经嵴细胞如何分化为不同的细胞类型是脊椎动物生物学研究中的一个基本问题。在这里，我们使用单细胞RNA测序的特点与神经嵴细胞的发展在迁移的早期阶段在斑马鱼躯干的转录变化。我们发现，神经嵴细胞是转录多样性和识别迁移前的人口已经表达的基因与分化的衍生物，特别是在xanthophore谱系。此外，我们在数据中识别出一群Rohon-Beard神经元。所提出的数据确定了多个躯干神经嵴细胞群和Rohon-Beard神经元的新的遗传标记，为以前未表征的脊椎动物发育关键基因提供了深入了解。
The neural crest is a migratory population of stem-like cells that contribute to multiple traits including the bones of the skull, peripheral nervous system, and pigment. How neural crest cells differentiate into diverse cell types is a fundamental question in the study of vertebrate biology. Here, we use single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. We show that neural crest cells are transcriptionally diverse and identify pre-migratory populations already expressing genes associated with differentiated derivatives, specifically in the xanthophore lineage. Further, we identify a population of Rohon–Beard neurons in the data. The data presented identify novel genetic markers for multiple trunk neural crest cell populations and Rohon–Beard neurons providing insight into previously uncharacterized genes critical for vertebrate development.
Notch信号传导下游PRDM1A和OLIG4在神经板边界处调节细胞命运。
影响因子：
2.7
作者：
Hernandez-Lagunas L;Powell DR;Law J;Grant KA;Artinger KB
通讯作者：
Artinger KB