Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESI/APCI-MS/MS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature
Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESI/APCI-MS/MS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature
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UPLC-ESI/APCI-MS/MS定量分析泽泻中8种三萜类化合物和2种倍半萜类化合物及其在最佳采收期和粗加工温度优化中的应用
DOI：
10.1155/2019/8320171
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发表时间：
2019-08-14
影响因子：
2.6
通讯作者：
Huang, Mingqing
中科院分区：
文献类型：
--
作者：
Tai, Yanni;Zou, Fuxian;Huang, Mingqing
泽泻为泽泻属植物泽泻属植物，中国俗称泽泻。Juzep(泽泻科)，一种中草药，用于治疗高脂血症、糖尿病、高血压、排尿困难和炎症。本研究建立了一种基于电喷雾电离(ESI)和常压化学电离(APCI)串联质谱仪(UPLC-ESI/APCIMS/MS)联用的灵敏可靠的方法，可同时测定RA中8种三萜类化合物(ESI模式)和2种倍半萜类化合物(APCI模式)。采用Waters公司的CORTECS UPLC C18色谱柱(200 mm x 2.1 m，1.6µm)，用水(含0.1%甲酸)和乙腈在7min内进行梯度洗脱，分析了10种标志物。建立的方法进行了线性、日内和日间精密度、准确度、回收率和稳定性的验证。10种标志物的校正曲线呈良好的线性回归(r>；0.9971)。方法检出限为0.14~1.67 ng/m L，定量限为0.44~5.65 ng/m L。日内和日间精密度的相对标准偏差(RSD，%)和精密度(RE，%)分别小于3.83%和1.21%和3.22%和1.46%，实际样品的重复性和稳定性分别小于2.78%和3.19%。10种标志物的回收率在97.24%~102.49%之间，RSD<4.05%。所建立的方法能有效地测定维甲酸中的10种标志物，并用于优化采收时间和原油加工温度。结果表明，90%枯萎期和70℃(或更低)可能是RA的最佳采收期和加工温度。
Rhizoma Alismatis (RA), widely known as "Ze-Xie" in China, is the tuber of Alisma orientale (Sam.) Juzep (Alismaceae), a Chinese herbal medicine that has been used to treat hyperlipidemia, diabetes, hypertension, dysuria, and inflammation. In this study, a sensitive and reliable method based on an ultra-performance liquid chromatography (UPLC) couple with two ionisation modes, including electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) tandem mass spectrometry (MS), namely, UPLC-ESI/APCI-MS/MS was developed and validated to simultaneously determine 8 triterpenoids (ESI mode) and 2 sesquiterpenoids (APCI mode) in RA. Ten marker compounds were analysed with a Waters' CORTECS UPLC C18 column (200 mm x 2.1 m, 1.6 mu m) and gradient elution with water (contained 0.1% formic) and acetonitrile within 7 min. The established method was validated for linearity, intra- and interday precisions, accuracy, recovery, and stability. The calibration curve for 10 marker compounds showed good linear regression (r > 0.9971). The limits of detection and quantification for analytes were 0.14-1.67 ng/mL and 0.44-5.65 ng/mL, respectively. The relative standard deviations (RSD, %) and accuracy (RE, %) of intra- and interday precisions were less than 3.83% and 1.21% and 3.22% and 1.46%, repeatability and stability for real samples were less than 2.78% and 3.19%, respectively. All recoveries of the 10 marker compounds ranged from 97.24% to 102.49% with RSDs less than 4.05%. The developed method efficiently determined the 10 marker compounds in RA and was subsequently applied to optimise harvest time and crude processing temperature. The result indicated the 90% wilted phase and 70 degrees C (or lower) may be the best harvest time and the processing temperature of RA.