Human HeLa cell enzymes that remove phosphoglycolate 3'-end groups from DNA.
Human HeLa cell enzymes that remove phosphoglycolate 3'-end groups from DNA.
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人类 HeLa 细胞酶，可去除 DNA 中的磷酸乙醇酸 3 端基团。
DOI：
10.1093/nar/20.10.2573
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发表时间：
1992
影响因子：
14.9
通讯作者：
Jorgensen,TJ
中科院分区：
文献类型：
--
作者：
Winters,TA;Weinfeld,M;Jorgensen,TJ
我们已经从HeLa细胞中纯化了三种色谱上不同的人类酶活性，它们能够将博来霉素处理的DNA转化为E的底物。coliDNA聚合酶I。本研究中使用的博来霉素处理的DNA底物已通过32 P-后标记试验进行了表征，并显示含有3′-磷酸乙醇酸末端的链断裂，超过95%的可检测剂量依赖性病变。纯化的HeLa细胞酶显示能够从该底物中去除3′-磷酸乙醇酸。此外，3′-磷酸乙醇酸去除和核苷酸掺入酶已被确定具有II类AP核酸内切酶活性。这些酶缺乏3′-5 ′核酸外切酶活性，因此与核酸外切酶III-anE不同。大肠杆菌酶能去除3′-磷酸乙醇酸。
We have purified three chromatographically distinct human enzyme activities from HeLa cells, that are capable of converting bleomycin-treated DNA into a substrate forE. coliDNA polymerase I. The bleomycin-treated DNA substrate used in this study has been characterized via a32P-postiabeling assay and shown to contain strand breaks with 3′-phosphoglycolate termini as greater than 95% of the detectable dose-dependent lesions. The purified HeLa cell enzymes were shown to be capable of removing 3′-phosphoglycolates from this substrate. Also 3′-phosphoglycolate removal and nucleotide incorporation were enzyme have been determined to possess Class II AP endonuclease activity. The enzymes lack 3′ – 5′ exonuclease activity and are, therefore, dissimilar to exonuclease III—anE. colienzyme that can remove 3′-phosphoglycolate.