Alternative poly(A) site utilization during adenovirus infection coincides with a decrease in the activity of a poly(A) site processing factor.
Alternative poly(A) site utilization during adenovirus infection coincides with a decrease in the activity of a poly(A) site processing factor.
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腺病毒感染期间的替代性聚腺苷酸位点利用与聚腺苷酸位点加工因子活性的降低同时发生。
DOI：
10.1128/mcb.13.4.2411-2419.1993
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发表时间：
1993
影响因子：
5.3
通讯作者：
Nevins,JR
中科院分区：
文献类型：
--
作者：
Mann,KP;Weiss,EA;Nevins,JR
识别和处理前mRNA以创建聚(A)加成部位，这是mRNA生物发生中的一个必要步骤，在使用聚(A)部位的频率不同的情况下，也可以是一种调节事件。在腺病毒感染过程中发现了一例这样的病例。在感染的后期阶段，主要的晚期转录单位利用5个聚(A)位点来产生20多个不同的mRNAs。主要晚期转录单位的近一半也在病毒感染的早期阶段表达。在表达的早期阶段，L1聚(A)位点的使用频率是L3聚(A)位点的三倍。相比之下，在感染后期，L3站点的使用频率是L1站点的三倍。最近的实验表明，CF1加工因子对Poly(A)位点富含Gu的下游元件的识别可能是Poly(A)位点选择的速率决定步骤。我们证明了CF1与L1聚(A)位的相互作用不如CF1与L3聚(A)位的相互作用稳定。我们还发现，当腺病毒感染进入晚期时，CF1活性水平显著下降。我们认为，这种CF1活性的降低，加上与L1聚(A)位点相互作用的相对不稳定，导致腺病毒感染后期L1聚(A)位点的使用减少。
The recognition and processing of a pre-mRNA to create a poly(A) addition site, a necessary step in mRNA biogenesis, can also be a regulatory event in instances in which the frequency of use of a poly(A) site varies. One such case is found during the course of an adenovirus infection. Five poly(A) sites are utilized within the major late transcription unit to produce more than 20 distinct mRNAs during the late phase of infection. The proximal half of the major late transcription unit is also expressed during the early phase of a viral infection. During this early phase of expression, the L1 poly(A) site is used three times more frequently than the L3 poly(A) site. In contrast, the L3 site is used three times more frequently than the L1 site during the late phase of infection. Recent experiments have suggested that the recognition of the poly(A) site GU-rich downstream element by the CF1 processing factor may be a rate-determining step in poly(A) site selection. We demonstrate that the interaction of CF1 with the L1 poly(A) site is less stable than the interaction of CF1 with the L3 poly(A) site. We also find that there is a substantial decrease in the level of CF1 activity when an adenovirus infection proceeds to the late phase. We suggest that this reduction in CF1 activity, coupled with the relative instability of the interaction with the L1 poly(A) site, contributes to the reduced use of the L1 poly(A) site during the late stage of an adenovirus infection.