A cyclin-dependent kinase inhibitor, dacapo, is necessary for timely exit from the cell cycle during Drosophila embryogenesis
A cyclin-dependent kinase inhibitor, dacapo, is necessary for timely exit from the cell cycle during Drosophila embryogenesis
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DOI：
10.1016/s0092-8674(00)81819-x
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发表时间：
1996-12-27
期刊：
影响因子：
64.5
通讯作者：
Hariharan, IK
中科院分区：
文献类型：
--
作者：
deNooij, JC;Letendre, MA;Hariharan, IK
在筛选与Rap 1 GT3相互作用的基因时，我们已经确定了果蝇基因dacapo（dap），它是cdk抑制剂p21/p27家族的成员。不像哺乳动物cdk抑制剂研究到dale，dap是正常胚胎发育所必需的。Dacapo体外抑制细胞周期蛋白cdk活性。在眼睛发育过程中过表达dap会干扰细胞周期进程，并与视网膜母细胞瘤同源物（Rbf）和细胞周期蛋白E发生遗传相互作用。胚胎中DAP的表达与细胞退出细胞周期平行。DAP突变胚胎在发育过程中延迟正常细胞周期的退出;许多细胞完成额外的周期，随后变得静止。因此，dap在胚胎发生过程中起作用，以实现精确定时退出细胞周期。
In a screen for genes that interact with the Rap1 GTPase, we have identified a Drosophila gene, dacapo (dap), which is a member of the p21/p27 family of cdk inhibitors. Unlike mammalian cdk inhibitors studied to dale, dap is essential for normal embryonic development. Dacapo inhibits cyclin-cdk activity in vitro. Overexpressing dap during eye development interferes with cell cycle progression and interacts genetically with the retinoblastoma homolog (Rbf) and cyclin E. dap expression in embryos parallels the exit of cells from the cell cycle. dap mutant embryos delay the normal cell cycle exit during development; many cells complete an additional cycle and subsequently become quiescent. Thus, dap functions during embryogenesis to achieve a precisely timed exit from the cell cycle.