Phialocephala sphaeroides sp nov., a new species among the dark septate endophytes from a boreal wetland in Canada
Phialocephala sphaeroides sp nov., a new species among the dark septate endophytes from a boreal wetland in Canada
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DOI：
10.1139/b04-030
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发表时间：
2004-05-01
期刊：
影响因子：
--
通讯作者：
Currah, RS
中科院分区：
文献类型：
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作者：
Wilson, BJ;Addy, HD;Currah, RS
从生长在陡峭湿地-旱地交错带两侧的植物中分离出的暗隔根内生真菌包括Phialocephala fortinii Wang&Wilcox，Leptodontidium orchidicola Sigler&Currah，Heteroconium chaetspira(Grove)Ellis，以及一种迄今未描述的类似P.fortinii的真菌。从该种分离到6个菌株，它们的特征是：(1)在培养中产生橙黄色可扩散色素，(2)在含有溴甲酚紫的酪氨酸上引起黄移，(3)具有液化明胶的能力，(4)在5℃下长时间培养后，由排列在大球头上的菌丝形成透明的分生孢子。初生分生孢子为子弹状，直径为1-1.5微米×2-3微米；后续分生孢子为球形，直径为1-1.5微米。与Phialocephala fortinii和其他Phialocephala物种的小亚基和内部转录间隔区序列的比较支持将这六个独特的菌株归入一个新物种Phialocephala sphaeroides B.J.Wilson sp.11月系统发育分析还表明，球形拟青霉在皮肤科中属于软体动物类群。与在交错带两侧分离的Fortinii不同，球形P.sphaeroides只从高酸性、泥炭藓占优势的湿地生境中获得，而不从酸性较弱、杨树占优势的山地生境中的同一物种中获得。
Dark septate root endophytic fungi from plants growing on either side of an abrupt wetland-upland ecotone included isolates of Phialocephala fortinii Wang & Wilcox, Leptodontidium orchidicola Sigler & Currah, Heteroconium chaetospira (Grove) Ellis, and a hitherto undescribed fungus resembling P. fortinii. Six isolates of this species were recovered and were distinctive in (i) producing an orange-tan diffusible pigment in culture, (ii) causing a yellow colour shift on casamino acids medium containing bromocresol purple, (iii) having the ability to liquefy gelatin, and microscopically, (iv) forming hyaline conidia from phialides arranged in large spherical heads after prolonged incubation at 5 degreesC. First-formed or primary conidia are bullet shaped, 1-1.5 mum x 2-3 mum; subsequent conidia are spherical and 1-1.5 mum in diameter. Small subunit and internal transcribed spacer region sequence comparisons with P. fortinii and other Phialocephala species supported placing these six unique strains in a new species, Phialocephala sphaeroides B.J. Wilson sp. nov. Phylogenetic analyses also suggest that P. sphaeroides is affiliated with mollisioid taxa in the Dermateaceae. In contrast with P. fortinii, which was isolated on both sides of the ecotone, P. sphaeroides was obtained only from plants in the highly acidic, Sphagnum-dominated wetland habitat and not from the same species in the less acidic, aspen-dominated upland site.