The initial analysis of a serine proteinase gene (AccSp10) from Apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses
The initial analysis of a serine proteinase gene (AccSp10) from Apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses
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中华蜜蜂丝氨酸蛋白酶基因 (AccSp10) 的初步分析：可能参与蛹发育、先天免疫和非生物应激反应
DOI：
10.1007/s12192-017-0818-5
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发表时间：
2017-11-01
影响因子：
3.8
通讯作者：
Guo, Xingqi
中科院分区：
文献类型：
--
作者：
Gao, Lijun;Wang, Hongfang;Guo, Xingqi
丝氨酸蛋白酶在天然免疫和昆虫发育中起着重要作用。我们从中华蜜蜂（Apis cerana cerana）中克隆了一个丝氨酸蛋白酶基因，命名为AccSp 10。对蛹不同发育阶段的RT-qPCR和Western blot分析表明，AccSp 10可能参与蛹黑色素的形成，促进蛹的发育。在成年工人中，AccSp 10的表达通过模拟有害环境的处理而上调，例如存在家蚕败血症芽孢杆菌、不同温度（4、24和42 °C）、HgCl 2、H2 O2和百草枯;例外的是用VC（维生素C）处理，其不上调AccSp 10的表达。Western blot证实了结果。纸片扩散试验表明，重组AccSp 10对E.大肠杆菌细胞死亡过程中的刺激与有害物质（氯化汞，百草枯和枯烯氢过氧化物）。这些结果表明，AccSp 10可能参与中国蜜蜂蛹的发育和保护免受微生物和非生物危害。
Serine proteinases play important roles in innate immunity and insect development. We isolated a serine proteinase gene, designated AccSp10, from the Chinese honeybees (Apis cerana cerana). RT-qPCR and a Western blot analysis at different pupal development stages indicated that AccSp10 might be involved in melanin formation in pupae and promote pupal development. In adult workers, the expression of AccSp10 was upregulated by treatments mimicking harmful environments such as the presence of Bacillus bombysepticus, different temperatures (4, 24 and 42 °C), HgCl2, H2O2 and paraquat; the exception was treatment with VC (vitamin C), which did not upregulate AccSp10 expression. Western blot confirmed the results. A disc diffusion assay indicated that recombinant AccSp10 accelerated E. coli cell death during stimulation with harmful substances (HgCl2, paraquat and cumene hydroperoxide). These findings suggest that AccSp10 may be involved in the pupal development of Chinese honeybees and protection against microorganisms and abiotic harms.