KINETIC STUDIES ON INSOLUBLE CELLULOSE-CELLULASE SYSTEM
KINETIC STUDIES ON INSOLUBLE CELLULOSE-CELLULASE SYSTEM
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DOI：
10.1002/bit.260171003
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发表时间：
1975-01-01
影响因子：
3.8
通讯作者：
HUANG, AA
中科院分区：
文献类型：
--
作者：
HUANG, AA
在间歇式反应器中，研究了绿色木霉纤维素酶在不同底物浓度和3种酶水平下对不溶性无定形纤维素的酶解。反应在50 ℃下进行。C和pH 4.8。酶在接触时迅速吸附到固体上，然后随着反应的进行逐渐返回到液相。建立了一个动力学模型来解释实验观察结果，该模型考虑了快速吸附、随后的缓慢反应以及随后的产物抑制。所得方程成功地关联了高达70%转化率的数据。讨论了动力学参数的确定方法。
Enzymatic hydrolysis of insoluble amorphous cellulose by Trichoderma viride cellulase was investigated in a batch reactor at several substrate concentrations and 3 enzyme levels. The reactions were carried out at 50.degree. C and pH 4.8. Enzyme was rapidly adsorbed onto solids on contact, then gradually returned to the liquid phase as the reaction proceeded. A kinetic model that considered the fast adsorption which was followed by the slow reaction, and subsequent product inhibition was developed to interpret the experimental observations. The resulting equation successfully correlated the data for up to 70% conversion. The methods for determining the kinetic parameters are discussed.