Lymph flow, shear stress, and lymphocyte velocity in rat mesenteric prenodal lymphatics
Lymph flow, shear stress, and lymphocyte velocity in rat mesenteric prenodal lymphatics
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DOI：
10.1080/10739680600893909
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发表时间：
2006-10-01
期刊：
影响因子：
2.4
通讯作者：
Zawieja, David C.
中科院分区：
文献类型：
--
作者：
Dixon, J. Brandon;Greiner, Steven T.;Zawieja, David C.
目的：测定原位物理收缩淋巴管的淋巴细胞速度、淋巴收缩和剪应力。方法：采用高速摄像系统捕捉大鼠肠系膜淋巴制剂的多次收缩周期。分析图像以确定流体速度、体积流速、壁面剪切应力和逆行流动。结果：淋巴细胞密度和通量分别为326 ~ 35500个细胞/ μ L和206 ~ 2030个细胞/min。淋巴管物理收缩，平均直径91±9.0 μ m，振幅39%。淋巴速度随期收缩的方向和幅度而变化，平均为0.87 +/- 0.18，峰值为2.2 ~ 9.0 mm/s。速度与收缩周期的相位差接近180度。平均淋巴流量为13.95±5.27 μ L/h，有短暂的淋巴流量逆转。这导致平均剪切为0.64 +/- 0.14，峰值为4-12达因/厘米(2)。结论：高速淋巴细胞追踪为测量整个期相收缩过程中的淋巴细胞通量提供了空间和时间分辨率。泊泽维尔流是一个合理的模型，可用于估计瓣间淋巴段大部分收缩周期的壁剪应力。剪切速率较低，但与血管中的剪切速率相比，剪切速率的变化幅度很大。
Objective: To measure lymphocyte velocity, lymphatic contraction, and shear stress in physically contracting lymphatics in situ.Methods: A high-speed video system was used to capture multiple contraction cycles in rat mesenteric lymphatic preparations. The images were analyzed to determine fluid velocity, volume flow rate, wall shear stress, and retrograde flow.Results: Lymphocyte density and flux varied from 326 to 35,500 cells/mu L and 206 to 2030 cells/min, respectively. Lymphatics contracted physically, with a mean diameter 91 +/- 9.0 mu m and amplitudes of 39%. Lymph velocity varied with the phasic contractions in both direction and magnitude with an average of 0.87 +/- 0.18 and peaks of 2.2-9.0 mm/s. The velocity was similar to 180 degrees out of phase with the contraction cycle. The average lymph flow was 13.95 +/- 5.27 mu L/h with transient periods of flow reversal. This resulted in an average shear of 0.64 +/- 0.14 with peaks of 4-12 dynes/cm(2).Conclusions: High-speed lymphocyte tracking provided the spatial and temporal resolution to measure lymphocyte flux throughout the phasic contraction. Poiseuille flow was a reasonable model for estimating wall shear stress through most of the phasic contraction cycle of the intervalvular lymphatic segments. Shear rate was low but had large variations in magnitude compared to that seen in blood vessels.