Original In -vitro antioxidant, antimutagenic and cancer cell growth inhibition activities of Rhododendron arboreum leaves and flowers
Original In -vitro antioxidant, antimutagenic and cancer cell growth inhibition activities of Rhododendron arboreum leaves and flowers
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DOI：
10.1016/j.sjbs.2020.01.030
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发表时间：
2020-07-01
影响因子：
4.4
通讯作者：
Ahmad, Parvaiz
中科院分区：
文献类型：
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作者：
Gautam, Vandana;Sharma, Anket;Ahmad, Parvaiz
采用超高效液相色谱、氨基酸分析仪和气相色谱-质谱仪对杜鹃花叶和花的甲醇提取物的活性成分进行了研究。UHPLC法显示提取物中存在不同的多酚类物质。GC-MS分析结果表明，叶中含有20种植物化学成分，花中含有17种化学成分；氨基酸分析仪鉴定出叶中含有11种氨基酸，花中含有10种氨基酸。通过体外抗氧化活性、Ames抗突变活性和肿瘤细胞生长抑制活性的四甲基偶氮唑蓝(3-4，5-二甲基四氮唑-2，5-二苯基四氮唑)比色法检测提取物的生物活性。在脂质过氧化抑制实验中，MEL的抗氧化活性(95.32%±40.37%)高于MEF(77.09%±44.17%)，其IC50103.6µg/ml和MEF的IC50103.6µg/ml分别为271.17µg/ml。在NO清除实验中，MEF的活性为94.46±0.32%(IC50150.13)，MEL的活性为83.71±0.74%(IC50179.52)。以TA-98和TA-100两株鼠伤寒沙门氏菌为研究对象，考察了两种提取物对叠氮化钠、4-硝基-邻苯二胺和2-氨基荧致突变剂的抗突变活性。分析采用预孵化和共孵化两种模式。然而，这两种提取物对不同的已知诱变剂都有相当大的抗诱变活性，花的抑制率比叶更有效。提取物对人宫颈癌细胞系(HeLa)、乳腺癌细胞系(MCF7)和肺癌细胞系(A549)的生长也有明显的抑制作用。在HeLa和A549中，MEL的活性分别为64.62±22.65和75.08±61.68%，而MEL的活性分别为53.11±22.84和45.92±22.43%。MEL在HeLa和A549中的EC50值分别为232.76和155.38微克/毫升，而MEF在HeLa和A549中的EC50分别为395.50微克/毫升和660.26微克/毫升。MEF对MCF7细胞的细胞毒作用最强(84.93%±111.17%)，其次是MEL(73.57%±71.27%)，其EC50值分别为95.16g/ml和172.19µg/ml。提取物的生物活性可归因于精密仪器鉴定的植物成分，提取物的生物活性可归因于精密仪器鉴定的活性成分。
In the current investigation, the active principles of the methanol extracts ofRhododendron arboreumleaves (MEL) and flowers (MEF) were investigated with the help of ultra-high performance liquid chromatography (UHPLC), amino acid analyzer and gas chromatography mass spectrometry (GC-MS). UHPLC revealed different polyphenols present in the extracts. GC-MS identified 20 phytochemicals in leaves and 17 in the flowers, whereas, amino acid analyzer confirmed 11 amino acids in leaves and 10 in the flowers. The extracts were subjected to the investigation of biological activity through analysis of antioxidant activity in differentin vitroassays, antimutagenic activity in Ames assay and cancer cell growth inhibition activity by MTT (3-4,5 dimethylthiazol-2,5 diphenyltetrazolium bromide) assay. MEL showed higher antioxidant activity in lipid peroxidation inhibition assay (95.32 ± 0.37%) than MEF (77.09 ± 4.17%) with IC50103.6 µg/ml for MEL and 271.17 µg/ml for MEF. In nitric oxide scavenging assay, an activity of 94.46 ± 0.32% (IC50150.13) was observed in MEF followed by 83.71 ± 0.74% (IC50179.52) in MEL. The antimutagenic activity of both the extracts was evaluated against sodium azide, 4-nitro-O-phenylenediamine and 2-aminofluorene mutagens in TA-98 and TA-100 strains ofSalmonella typhimurium. The analysis was carried out using pre- and co-incubation modes. However, both extracts were observed to possess considerable antimutagenic activity against different known mutagens, flowers came out to be more effective than the leaves in terms of % inhibition. The extracts also exhibited significant cancer cell growth inhibition activity, when tested against 3 cancer cell lines namely, Human cervical cancer cell line (HeLa), Breast cancer cell line (MCF7) and Lung cancer cell line (A549). In case of HeLa and A549, MEL showed higher activity of 64.62 ± 2.65 and 75.08 ± 1.68% as compared to 53.11 ± 2.84 and 45.92 ± 2.43% in MEL, respectively. The EC50values for MEL in HeLa and A549 were noted to be 232.76 and 155.38 µg/ml, respectively, whereas, MEF had EC50of 395.50 µg/ml in HeLa and 660.26 µg/ml in A549. Further, MEF showed higher cytotoxicity in MCF7 cell line (84.93 ± 1.17%) followed by the MEL (73.57 ± 1.27%) with EC50value of 95.16 µg/ml for MEF followed by 172.19 µg/ml for MEL. The biological activities of the extracts can be attributed to the phyto-constituents identified by sophisticated instruments.The biological activities of the extracts can be attributed to the active principles identified by sophisticated instruments.