Photoperiod regulates growth, puberty and hypothalamic neuropeptide and receptor gene expression in female Siberian hamsters
Photoperiod regulates growth, puberty and hypothalamic neuropeptide and receptor gene expression in female Siberian hamsters
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DOI：
10.1210/en.141.12.4349
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发表时间：
2000-12-01
期刊：
影响因子：
4.8
通讯作者：
Mercer, JG
中科院分区：
文献类型：
--
作者：
Adam, CL;Moar, KM;Mercer, JG
在季节性哺乳动物中，生长和生殖轴都受光周期的调节。雌性西伯利亚仓鼠在长日照（LD）或短日照（SD）光周期中饲养长达12周，从3周龄断奶开始（实验1）。LD仓鼠具有特征性的更快的增长和更高的渐近体重，肥胖，和瘦素基因在脂肪组织中的表达。只有LD女性达到青春期。下丘脑弓状核瘦素受体（OB-Rb），POMC和黑皮质素S受体（MC 3-R）的基因表达在LD中较高，但在SD中与断奶水平相比没有变化。与此相反，在SD中，弓状核中可卡因和安非他明调节转录物（CART）的基因表达更高。在断奶后15周将SD雌性动物转移到LD（实验2）增加了体重、瘦素信号和POMC的基因表达，但未能诱导正常青春期开始或增加OB-Rb和MC 3-R的基因表达。因此，青春期的光周期调节可能受到年龄、光周期史、瘦素信号传导和瘦素敏感的下丘脑黑皮质素系统（POMC，MCS-R）活性的变化的调节。CART在光周期调节生长的作用建议，因为在CART基因表达的变化之前显着分歧的生长轨迹在相反的光周期。
In seasonal mammals, both the growth and reproductive axes are regulated by photoperiod. Female Siberian hamsters were kept, for up to 12 weeks, in long-day (LD) or short-day (SD) photoperiod, from weaning at 3 weeks of age (Exp 1). LD hamsters had characteristically faster growth and higher asymptotic body weight, adiposity, and leptin gene expression in adipose tissue. Only LD females attained puberty. Gene expression in the hypothalamic arcuate nucleus for leptin receptor (OB-Rb), POMC, and melanocortin S-receptor (MC3-R) was higher in LD but did not change from weaning levels in SD. In contrast, gene expression in the arcuate nucleus for cocaine and amphetamine-regulated transcript (CART) was higher in SD Transfer of SD females to LD at 15 weeks post weaning (Exp 2) increased body weight, leptin signal, and gene expression for POMC but failed to induce normal puberty onset or to increase gene expression for OB-Rb and MC3-R. Therefore, photoperiodic regulation of puberty may be modulated by age, by photoperiodic history, and by changes in leptin signaling and the activity of the leptin-sensitive hypothalamic melanocortin system (POMC, MCS-R). A role for CART in photoperiodic regulation of growth is suggested, because the changes in CART gene expression preceded significant divergence of growth trajectories in the opposite photoperiods.