Genomic characterization of two Chinese isolates of Porcine respiratory and reproductive syndrome virus
Genomic characterization of two Chinese isolates of Porcine respiratory and reproductive syndrome virus
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DOI：
10.1007/s00705-004-0292-0
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Yang, HC
中科院分区：
文献类型：
--
作者：
Gao, ZQ;Guo, X;Yang, HC
对猪呼吸与繁殖综合征病毒（Porcine respiratory and reproductive syndrome virus，PRRSV）中国分离株HB-1（sh）/2002和HB-2（sh）/2002进行了全基因组测序和分析。HB-1（sh）/2002和HB-2（sh）/2002的基因组大小分别为15，411和15，373个核苷酸，不包括poly（A）尾。与中国分离株BJ-4、北美分离株VR 2332和欧洲分离株基因组序列的比较分析（莱利斯塔病毒，LV）的同源性分析表明，HB-1（sh）/2002与BJ-4和VR 2332的同源性为89.8%，而与LV的同源性仅为54.7%; HB-2（sh）/2002与BJ-4和VR 2332的同源性分别为89.4%和89.5%，与LV的同源性为54.3%，表明这两个新分离株与北美PRRSV基因型有关。基于结构蛋白ORF的核苷酸序列的系统发育分析表明，这两个新的中国分离物属于同一个遗传亚群。HB-2（sh）/2002与其他北美PRRSV分离株相比，在NSP 2的ORF 1编码的非结构蛋白和ORF 3编码的结构蛋白GP 3上也存在变异，即在NSP 2上发现了12个氨基酸的缺失，在GP 3上发现了1个氨基酸的缺失。因此，HB-2（sh）/2002是一个具有独特缺失的新菌株。
The genomes of two isolates of Porcine respiratory and reproductive syndrome virus (PRRSV) from China, designated HB-1(sh)/2002 and HB-2(sh)/2002, were sequenced and analyzed. The size of the genomes of HB-1(sh)/2002 and HB-2(sh)/2002 were 15,411 and 15,373 nucleotides respectively, excluding the poly(A) tails. Comparative analysis with the genomic sequences of another Chinese isolate (BJ-4) and North American (VR2332) and European (Lelystad virus, LV) viruses revealed that HB-1(sh)/2002 shared 89.8% identity with BJ-4 and VR2332, but only 54.7% with LV; while HB-2(sh)/2002 shared 89.4% and 89.5% identity with BJ-4 and VR2332 respectively and 54.3% with LV, indicating that the two new Chinese isolates were related to the North American PRRSV genotype. Phylogenetic analysis based on the nucleotide sequence of the structural protein ORF's showed that the two new Chinese isolates belong to same genetic subgroup. HB-2(sh)/2002 additionally exhibited variations in the NSP2 nonstructural protein encoded by ORF1 and the structural protein GP3 encoded by ORF3 in comparison with other North American PRRSV isolates, namely a 12 amino acids deletion in Nsp2 and one amino acid deletion in GP3 were found in HB-2(sh)/2002. Therefore, HB-2(sh)/2002 was a novel strain with unique deletions.