Angiogenesis and cell proliferation in human craniopharyngioma xenografts in nude mice.
Angiogenesis and cell proliferation in human craniopharyngioma xenografts in nude mice.
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DOI：
10.3171/ped.2006.105.4.306
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发表时间：
2006-10
影响因子：
4.1
通讯作者：
Jianguo Xu;C. You;Sizhong Zhang;Si-qing Huang;Bo-wen Cai;Zhenggui Wu;Hao Li
中科院分区：
文献类型：
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作者：
Jianguo Xu;C. You;Sizhong Zhang;Si-qing Huang;Bo-wen Cai;Zhenggui Wu;Hao Li
目的颅咽管瘤是鞍区和鞍上区最常见的先天性肿瘤之一，占颅内肿瘤的4% ~ 6%。其肿瘤发生和生物学行为尚未得到很好的研究，也没有建立细胞系或动物模型。为了更好地了解这种肿瘤，提高临床治疗水平，作者将人肿瘤细胞植入胸腺裸鼠体内，研究了皮下颅咽管瘤异种移植物的血管生成和细胞增殖情况。方法将手术标本中提取的人颅咽管瘤细胞皮下植入BALB/c-nu/nu裸鼠，建立移植瘤动物模型。采用链霉亲和素-过氧化物酶复合物免疫组化染色鉴定细胞表型，评价异种移植物的血管生成和增殖。通过计算机辅助显微镜定量检测细胞角蛋白、小染色体维持缺陷6 （MCM6）蛋白和内皮细胞标志物CD34在异种移植切片上的表达。15只裸鼠获得27块存活的皮下异种移植物。总着床成功率28.12%，其中金刚质上皮瘤（AE） 37.50%，鳞状乳头状瘤（SPT） 18.75%。在所有异种移植物中均观察到毛细血管的形成和细胞增殖。存活移植物微血管密度与MCM6免疫染色程度呈正相关（r = 0.410, p 0.05）。结论采用异位植入法在裸鼠体内建立了人颅咽管瘤动物模型。存活的异种移植物维持其血管形成和增殖活动，直到12周收获。
OBJECT Craniopharyngioma is one of the most common congenital tumors of the sellar and suprasellar regions and accounts for between 4 and 6% of all intracranial tumors. Its oncogenesis and biological behavior have not been well studied, and neither a cell line nor an animal model have been established. To better understand the tumor and improve its clinical management, the authors investigated the angiogenesis and cellular proliferation in subcutaneous craniopharyngioma xenografts obtained by implanting human tumor cells into athymic nude mice. METHODS Human craniopharyngioma cells obtained from surgical specimens were subcutaneously implanted into BALB/c-nu/nu nude mice to establish a preliminary animal model of a transplanted tumor. Immunohistochemical staining with streptavidin-peroxidase complex was used to identify the cell phenotype and to evaluate the angiogenesis and proliferation in the xenografts. Expression of cytokeratin, minichromosome maintenance deficient 6 (MCM6) protein, and endothelial cell marker CD34 on the xenograft sections were assayed quantitatively by computer-assisted microscopy. Twenty-seven surviving subcutaneous xenografts were obtained in 15 nude mice. The total implantation success rate was 28.12% (adamantine epithelioma [AE], 37.50%; squamous papillary tumor [SPT], 18.75%). Formation of capillaries and cell proliferation were observed in all of these xenografts. Microvessel density and degree of MCM6 immunostaining were positively correlated in the surviving grafts (r = 0.410, p 0.05). CONCLUSIONS A preliminary animal model of human craniopharyngioma was established in the nude mouse by heterotopic implantation. Surviving xenografts maintained their vascularization and proliferation activities until harvesting at 12 weeks.