Identification and Quantification of Aconitines and Colchicine in Serum, Urine, and Plants using MonoSpin C18 and LC-MSMS
Identification and Quantification of Aconitines and Colchicine in Serum, Urine, and Plants using MonoSpin C18 and LC-MSMS
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DOI：
10.1007/s10337-015-2921-z
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发表时间：
2015-08-01
期刊：
影响因子：
1.7
通讯作者：
Inokuchi, Sadaki
中科院分区：
文献类型：
--
作者：
Saito, Takeshi;Umebachi, Rimako;Inokuchi, Sadaki
乌头属和秋水仙属是已知的高毒性植物，基于它们各自的毒素，即乌头和秋水仙碱。毒砂和秋水仙碱中毒通常是由于误认和污染而发生的，也与杀人和自杀案件有关。检测体液和剩饭剩菜中乌头碱类和秋水仙碱类药物对天然毒物引起的疑似食物中毒的临床和法医毒理学调查具有重要意义。因此，我们已经开发和验证了快速测定乌头碱，次乌头碱，耶乌头碱，中乌头碱，秋水仙碱在血清，尿液和植物中使用液相色谱-串联质谱。使用C-18整体旋转柱从血清和尿液中提取所有目标化合物以及内标物。以0.1、0.8、6和18 ng mL（-1）浓度加标的血清中所有化合物的回收率范围为96.8%至101.4%。以0.1、8、80和190 ng mL（-1）浓度加标的尿液中所有化合物的回收率范围为96.6%至101.9%。评价的所有化合物的试验内和试验间准确度和精密度（表示为相对标准偏差，%RSD）分别在96.7- 103.2%和1.7- 11.5%范围内。乌头碱和秋水仙碱的检测限和定量限分别为0.05和0.1 ng/mL（-1）。该方法的色谱运行时间为7.0 min。血清和尿液的校准曲线范围分别为0.1 - 20 ng/mL（血清）和0.1-200 ng/mL（尿液）。该方法已成功应用于临床乌头碱和秋水仙碱中毒病例。
Aconitum and Colchicum are known to be highly toxic plants based on their respective toxins, namely aconite and colchicine. Aconite and colchicine poisoning generally occurs because of misidentification and contamination, and is also associated with homicide and suicide cases. The detection of aconitines and colchicine in body fluids and leftovers plays an important role in clinical and forensic toxicology investigation of suspected food poisoning by natural poisons. Therefore, we have developed and validated a rapid determination of aconitine, hypaconitine, jesaconitine, mesaconitine, and colchicine in serum, urine, and plants using liquid chromatography-tandem mass spectrometry. All targeted compounds, together with internal standards, were extracted from serum and urine using a C-18 monolithic spin column. Recovery of all compounds from serum, spiked at concentrations of 0.1, 0.8, 6, and 18 ng mL(-1), ranged from 96.8 to 101.4 %. Recovery of all compounds from urine, spiked at concentrations of 0.1, 8, 80 and 190 ng mL(-1), ranged from 96.6 to 101.9 %. The intra- and inter-assay accuracy and precision (expressed as relative standard deviation, %RSD) evaluated for all compounds were within 96.7-103.2 % and 1.7-11.5 %, respectively. Detection limits and quantitation limits of aconitines and colchicine for serum and urine were 0.05 and 0.1 ng mL(-1), respectively. This method had a chromatographic run time of 7.0 min. Calibration curves for serum and urine ranged from 0.1 to 20 ng mL(-1) for serum and 0.1-200 ng mL(-1) for urine, respectively. The validated method was successfully applied to clinical aconitine and colchicine poisoning cases.