Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples
Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples
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DOI：
10.1117/1.3556722
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发表时间：
2011-03-01
影响因子：
3.5
通讯作者：
Riches, Andrew C.
中科院分区：
文献类型：
--
作者：
Canetta, Elisabetta;Mazilu, Michael;Riches, Andrew C.
标准拉曼光谱（SRS）是一种用于生物医学领域区分正常细胞和癌细胞的非侵入性技术。然而，强荧光背景的存在影响了SRS在实时临床应用中的应用。最近，我们报道了一种新的调制拉曼光谱（MRS）技术从背景中提取拉曼光谱。在本文中，我们首次将MRS应用于尿液样本中人类尿路上皮细胞（SV-HUC-1）和膀胱癌细胞（MGH）的鉴定。这些结果与SRS得到的结果进行了比较。使用主成分分析的分类清楚地表明，MRS可以区分SV-HUC-1和MGH细胞的拉曼光谱，具有高灵敏度（98%）和特异性（95%）。在暴露于尿液长达6小时后，MRS也用于区分SV-HUC-1和MGH细胞。我们观察到SV-HUC-1和MGH细胞的MRS随尿液中时间的显著变化，表明样本收集条件对于将该方法应用于临床尿液样本将是重要的。(C) 2011光学仪器工程师学会（SPIE）(DOI: 10.1117/1.3556722)
Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows that MRS allows discrimination between Raman spectra of SV-HUC-1 and MGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h. We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3556722]