INCREASES IN ACTIVATED T-LYMPHOCYTES, EOSINOPHILS, AND CYTOKINE MESSENGER-RNA EXPRESSION FOR INTERLEUKIN-5 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IN BRONCHIAL BIOPSIES AFTER ALLERGEN INHALATION CHALLENGE IN ATOPIC ASTHMATICS
INCREASES IN ACTIVATED T-LYMPHOCYTES, EOSINOPHILS, AND CYTOKINE MESSENGER-RNA EXPRESSION FOR INTERLEUKIN-5 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IN BRONCHIAL BIOPSIES AFTER ALLERGEN INHALATION CHALLENGE IN ATOPIC ASTHMATICS
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DOI：
10.1165/ajrcmb/8.1.35
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发表时间：
1993-01-01
影响因子：
6.4
通讯作者：
DURHAM, SR
中科院分区：
文献类型：
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作者：
BENTLEY, AM;MENG, Q;DURHAM, SR
采用免疫组织学和原位杂交技术评价人过敏原诱导哮喘支气管黏膜细胞的存在、激活状态和细胞因子mRNA谱。15名特应性哮喘患者接受过敏原和过敏原稀释剂的吸入挑战，以随机顺序进行，间隔至少3周。攻毒后24 h行支气管活检。免疫染色显示，与稀释液相比，过敏原刺激后分泌嗜酸性粒细胞（EG2+, P < 0.05）和白细胞介素-2受体（IL-2R）阳性细胞（CD25+, P < 0.01）的数量增加。在总白细胞（CD45+）、T淋巴细胞（CD3+、CD4+和CD8+）、弹性酶阳性中性粒细胞、巨噬细胞（CD68+）或肥大细胞亚型(MC（T)+或MC(TC)+）的数量上没有观察到差异。原位杂交显示，与稀释液相比，过敏原刺激后表达IL-5 mRNA （P < 0.02）和粒细胞/巨噬细胞集落刺激因子mRNA （P < 0.01）的细胞数量显著增加。表达IL-4 mRNA的细胞数量与表达干扰素- γ mRNA的细胞数量呈显著负相关（r = -0.75, P < 0.02）。结果支持细胞因子可能来自活化T淋巴细胞的观点，可能有助于过敏性哮喘期间局部嗜酸性粒细胞的积累。
Immunohistology and in situ hybridization were used to evaluate the presence, activation status, and cytokine mRNA profile of cells in the bronchial mucosa during human allergen-induced asthma. Fifteen atopic asthmatic subjects underwent inhalation challenge with allergen and with allergen diluent, performed in random order separated by an interval of at least 3 wk. Bronchial biopsies were obtained 24 h after challenge. Immunostaining revealed increases in the numbers of secreting eosinophils (EG2+; P < 0.05) and in interleukin-2 receptor (IL-2R)-positive cells (CD25+; P < 0.01) after allergen compared with diluent challenge. No differences were observed in the numbers of total leukocytes (CD45+), T lymphocytes (CD3+, CD4+, and CD8+), elastase-positive neutrophils, macrophages (CD68+), or mast cell subtypes (MC(T)+ or MC(TC)+). In situ hybridization revealed significant increases in the numbers of cells expressing mRNA for IL-5 (P < 0.02) and granulocyte/macrophage colony-stimulating factor (P < 0.01) after allergen compared with diluent challenge. A significant inverse relationship was observed between the number of cells expressing mRNA for IL-4 and for interferon-gamma (r = -0.75, P < 0.02). The results support the view that cytokines possibly from activated T lymphocytes may contribute to local eosinophil accumulation during allergen-induced asthma.