Bioinformatics analysis integrating metabolomics of m6A RNA microarray in intervertebral disc degeneration.
Bioinformatics analysis integrating metabolomics of m6A RNA microarray in intervertebral disc degeneration.
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椎间盘退变中 m6A RNA 微阵列代谢组学的生物信息学分析。
DOI：
10.2217/epi-2020-0101
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发表时间：
2020-07
期刊：
影响因子：
3.8
通讯作者：
Fuxin Wei
中科院分区：
文献类型：
--
作者：
Xiaoshuai Wang;Ningning Chen;Zefeng Du;Zemin Ling;Penghui Zhang;Jiaming Yang;Mohammed Khaleel;Anthony N Khoury;Jianwen Li;Songbo Li;Haoyang Huang;Xinwei Zhou;Yueyin Han;Fuxin Wei
Aim: To explore the potential functions and mechanism of N6.methyladenosine (m6A) abnormality of RNAs in nucleus pulposus from the intervertebral disc degeneration (IDD). Materials & methods: We performed rat model, m6A epitranscriptomic microarray, bioinformatics analysis and metabolomics. Results: In IDD, most of the differentially methylated RNAs showed a significant demethylation situation. The competing endogenous RNA network LOC102555094/miR-431/GSK-3β combining downstream Wnt pathway were identified in bioinformatics analysis. For metabolomics, activation of Wnt pathway led to reprogramming of glucose metabolism and enzyme activation of PKM2. Finally, quantitative real-time PCR and methylated RNA immunoprecipitation coupled with quantitative real-time PCR revealed the positive correlation between demethylation of LOC102555094 and expression of both FTO and ZFP217. Conclusion: LOC102555094 might be demethylated by ZFP217, activating FTO and LOC102555094/miR-431/GSK-3β/Wnt played a crucial role in IDD.
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影响因子：
23.9
作者：
Weng H;Huang H;Wu H;Qin X;Zhao BS;Dong L;Shi H;Skibbe J;Shen C;Hu C;Sheng Y;Wang Y;Wunderlich M;Zhang B;Dore LC;Su R;Deng X;Ferchen K;Li C;Sun M;Lu Z;Jiang X;Marcucci G;Mulloy JC;Yang J;Qian Z;Wei M;He C;Chen J
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20.3
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R. Su;Zejuan Li;Hengyou Weng;X. Weng;Zhixiang Zuo;Chenying Li;Huilin Huang;Chao Hu;Xi Qin;L. Tang;Yungui Wang;Gia-ming Hong;Hao Huang;Xiao Wang;Ping Chen;S. Gurbuxani;Stephen Arnovitz;Yuanyuan Li;Shenglai Li;J. Strong;M. E. Neilly;R. Larson;Xi Jiang;Pumin Zhang;Jie Jin;Chuan He;Jianjun Chen
影响因子：
3
作者：
VIDEMAN, T;SAMA, S;GIBBONS, L
通讯作者：
GIBBONS, L
影响因子：
3.7
作者：
Liu G;Cao P;Chen H;Yuan W;Wang J;Tang X
通讯作者：
Tang X
CircSEMA4B 靶向 miR-431 通过 Wnt 通路调节椎间盘退变中 IL-1 β 诱导的髓核细胞降解变化
DOI：
10.1016/j.bbadis.2018.08.033
发表时间：
2018-11-01
影响因子：
6.2
作者：
Wang, Xiaobin;Wang, Bing;Lu, Chang
通讯作者：
Lu, Chang