Odontoblast marker gene expression is enhanced by a CC-chemokine family protein MIP-3α in human mesenchymal stem cells
Odontoblast marker gene expression is enhanced by a CC-chemokine family protein MIP-3α in human mesenchymal stem cells
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DOI：
10.1016/j.archoralbio.2007.04.004
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发表时间：
2007-10-01
影响因子：
3
通讯作者：
Ueda, M.
中科院分区：
文献类型：
--
作者：
Iejima, D.;Sumita, Y.;Ueda, M.
目的：巨噬细胞炎症蛋白-3 α (MIP-3 α) 是一种主要的 CC 趋化因子家族蛋白，可作为间充质细胞（包括成骨细胞和牙髓细胞）的分化因子。本研究的目的是探讨MIP-3α对人间充质干细胞体外分化的影响。设计：在存在或不存在 MIP-3 α 以及存在或不存在成骨因子（地塞米松、β-甘油磷酸和抗坏血酸）的情况下，将人间充质干细胞保存在 Dulbecco 改良的 Eagle 培养基中。测量碱性磷酸酶（ALP）活性，并通过 RT-PCR 和蛋白质印迹检查成牙本质细胞和成骨细胞标志物的表达。结果：与对照组相比，单独的 MIP-3 α 不会增加 ALP 活性。 MIP-3α和成骨因子的组合增加了ALP活性，超过了单独使用成骨因子所观察到的增加。只有在第 7 天将 MIP-3 α 与成骨因子一起添加到四分之三的样品中时，才能检测到成牙本质细胞标记物 dspp 的 mRNA 表达。仅在第 5 天之前用 MIP-3 α 和成骨因子处理的样品中 DSP 蛋白水平有所增加。相反，MIP-3 α 不影响成骨细胞标志物 CBFA1 或 BSP 的水平。结论：本研究表明，MIP-3 α 增强了成牙本质细胞相关基因的基因表达和蛋白水平，而不影响成骨蛋白 CBFA1 或 BSP 的水平。 (C) 2007 Elsevier Ltd. 保留所有权利。
Objective: Macrophage inflammatory protein-3 alpha (MIP-3 alpha) is a major CC-chemokine family protein, which serves as a differentiation factor for mesenchymal cells, including osteoblasts and dental pulp cells. The purpose of this study was to investigate the influence of MIP-3 alpha on human mesenchymal stem cell differentiation in vitro. Design: Human mesenchymal stem cells were maintained in Dulbecco's modified Eagle's medium in the presence or absence of MIP-3 alpha and the presence or absence of osteogenic factors (dexamethasone, beta-glycerophoshate and ascorbic acid). Alkaline phosphatase (ALP) activity was measured, and expression of odontoblast and osteoblast markers were examined by RT-PCR and Western blotting. Results: MIP-3 alpha alone did not increase ALP activity, as compared to controls. The combination of MIP-3 alpha and osteogenic factors increased ALP activity beyond increases observed with osteogenic factors alone. mRNA expression of the odontoblast marker dspp was only detectable when MIP-3 alpha was added together with osteogenic factors at day 7 in three out of four samples. DSP protein level was increased only in the samples treated with both MIP-3 alpha and osteogenic factors until day 5. In contrast, MIP-3 alpha did not influence levels of the osteoblast markers CBFA1 or BSP. Conclusions: The present study demonstrated that MIP-3 alpha enhanced gene expression and protein levels of odontoblast-related genes, without affecting levels of the osteogenic proteins CBFA1 or BSP. (C) 2007 Elsevier Ltd. All rights reserved.