Nucleotide Excision Repair Gene Subunit XPD is Highly Expressed in Cervical Squamous Cell Carcinoma
Nucleotide Excision Repair Gene Subunit XPD is Highly Expressed in Cervical Squamous Cell Carcinoma
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核苷酸切除修复基因亚基XPD在宫颈鳞状细胞癌中高表达
DOI：
10.1007/s12253-012-9527-7
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发表时间：
2012
影响因子：
2.8
通讯作者：
Huaizeng Chen
中科院分区：
文献类型：
--
作者：
F. Ye;Jie Jiao;Cai;Q. Cheng;Huaizeng Chen
哺乳动物转录因子II H复合物中的亚基之一XPD（TFIIH p80）在核苷酸切除修复途径中起重要作用。异常DNA切除修复等事件可能参与宫颈癌的发生过程。应用免疫组织化学方法检测84例正常宫颈组织和148例宫颈鳞癌组织中XPD蛋白的表达。另外，采用qRT-PCR方法分析69例新鲜正常宫颈组织和110例宫颈癌组织中XPD mRNA的表达。分析XPD表达与患者年龄、FIGO分期、肿瘤大小、间质侵犯、淋巴结转移及组织学分级的关系。XPD（TFIIH p80）蛋白主要在细胞质中检测到。我们发现宫颈癌与正常宫颈组织中XPD表达水平存在统计学显著差异（Z =-7.302，P = 0.000）。XPD mRNA在宫颈癌组织中的表达显著高于正常宫颈组织（t = 6.942，P = 0.000）。而XPD表达与患者年龄、FIGO分期、肿瘤大小、间质浸润、淋巴结转移及组织学分级无明显相关性（P = 0.089，0.953，0.809，0.275，0.421，0.387）。我们的研究结果表明，XPD在宫颈鳞癌组织中高表达。在XPD转录过程中可能会发生一种知之甚少的变化，导致从mRNA到蛋白质水平的异常富集，从而使蛋白质无法执行切除修复的正常功能。有必要进一步研究，以阐明所涉及的具体机制。
One of the subunits in the mammalian transcription factor II H complex, XPD (TFIIH p80), plays a significant role in the nucleotide excision repair pathway. Events such as abnormal DNA excision repair may be involved in the cervical carcinogenesis process. Expression of the human XPD protein was examined using immunohistochemistry in 84 normal cervical tissues and 148 cervical squamous cell carcinoma samples. Additionally, qRT-PCR was performed to analyse the XPD mRNA expression in 69 fresh normal cervical tissues and 110 cervical carcinoma samples. The relationships between XPD expression and various clinicopathological features (including age, FIGO stage, tumor size, stroma involvement, lymph node metastasis and histologic grade) were assessed. The XPD (TFIIH p80) protein was detected primarily in the cytoplasm. We found a statistically significant difference in XPD expression level in cervical carcinoma versus normal cervical tissue (Z = −7.302, P = 0.000). Notably, XPD mRNA was significantly over-expressed in cervical carcinoma tissues but not in normal cervix tissues (t = 6.942, P = 0.000). However, no statistically significant relationship was found regarding XPD expression and age, FIGO stage, tumor size, stroma involvement, lymph node metastasis or histologic grade (P = 0.089, 0.953, 0.809, 0.275, 0.421, 0.387 respectively). Our results showed that XPD was highly expressed in cervical squamous cell carcinoma tissues. A poorly understood change may occur during the XPD transcription process, resulting in the abnormal enrichment seen from mRNA to the protein level, thus leaving the protein unable to perform the normal function of excision repair. There is a need for further research in order to elucidate the specific mechanism involved.
影响因子：
4.7
作者：
Sturgis, EM;Zheng, R;Wei, QY
通讯作者：
Wei, QY