Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity.
Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity.
复制标题
DOI：
10.1017/s0016672309990140
复制
发表时间：
2009-08
影响因子：
1.5
通讯作者：
Y. Yoshida;M. Honjo;N. Kitamoto;R. Ohsawa
中科院分区：
文献类型：
--
作者：
Y. Yoshida;M. Honjo;N. Kitamoto;R. Ohsawa
大花报春Morren是一种多年生克隆草本植物，广泛分布于日本，但在野外面临灭绝的危险。在以前的研究中，我们揭示了物种的遗传多样性，利用叶绿体和核DNA，并利用这些信息来定义保护单位。然而，我们缺乏关于适应性遗传多样性的信息，这对长期生存和保护单位的定义都很重要。为了确定适应性性状，表现出适应性分化的群体之间，我们研究了6个数量性状的遗传变异内和群体之间的3年，在一个共同的花园，使用110个基因从5个自然群体来自日本的3个地区。花芽分化天数是一个适应性数量性状，群体间遗传分化程度（QST）远大于8个微卫星标记（FST）。该性状与环境因子之间的关系表明，在每个栖息地的生长期间的平均温度，芽的起始天数呈负相关。这表明，在芽分化之前延迟的适应性分化是由生境之间的温度差异导致的选择压力引起的。我们的研究结果表明，基于适应多样性和中性遗传多样性，琦玉人口代表了一个新的保护单位。
Primula sieboldii E. Morren is a perennial clonal herb that is widely distributed in Japan, but in danger of extinction in the wild. In a previous study, we revealed the genetic diversity of the species using chloroplast and nuclear DNA and used this information to define conservation units. However, we lacked information on adaptive genetic diversity, which is important for long-term survival and, thus, for the definition of conservation units. In order to identify adaptive traits that showed adaptive differentiation among populations, we studied the genetic variation in six quantitative traits within and among populations for 3 years in a common garden using 110 genets from five natural populations from three regions of Japan. The number of days to bud initiation was adaptive quantitative trait for which the degree of genetic differentiation among populations (QST) was considerably larger than that in eight microsatellite markers (FST). The relationship between this trait and environmental factors revealed that the number of days to bud initiation was negatively correlated, with the mean temperature during the growing period at each habitat. This suggests that adaptive differentiation in the delay before bud initiation was caused by selective pressure resulting from temperature differences among habitats. Our results suggest that based on adaptive diversity and neutral genetic diversity, the Saitama population represents a new conservation unit.