Transcription factor 7-like 2-associated signaling mechanism in regulating cementum generation by the NF-kappa B pathway
Transcription factor 7-like 2-associated signaling mechanism in regulating cementum generation by the NF-kappa B pathway
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转录因子 7 样 2 相关信号机制通过 NF-κ B 途径调节牙骨质生成
DOI：
10.1002/jcp.28685
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发表时间：
2019
影响因子：
5.6
通讯作者：
Xia Haibin
中科院分区：
文献类型：
--
作者：
Zhao Zifan;Dai Jing;Yin Chengcheng;Wang Xuzhu;Wang Jinyang;Jia Xiaoshi;Zhao Qin;Fu Hui;Zhang Yufeng;Xia Haibin
牙骨质再生是牙周组织工程和再生中重要且具有挑战性的阶段。包括牙骨质在内的牙周组织的病理变化对于糖尿病、白血病、获得性免疫缺陷综合症等全身性疾病的精准诊断和顽固治疗具有重要意义。在这里，我们发现在牙周组织发育过程中，转录因子7样2（Tcf7l2）在牙周组织和牙囊中广泛表达。在小鼠成牙骨质细胞系 (OCCM-30) 中，当 Tcf7l2 基因沉默时，NF-κB 的激活和成牙骨质细胞矿化显着降低。此外，Tcf7l2 对 NF-κB 和成牙骨质细胞矿化具有积极作用。因此，Tcf7l2 通过 NF-κB 途径促进牙骨质形成。此外，我们发现Tcf7l2fl/fl小鼠中磷酸化p65的表达降低和牙骨质薄层。这些结果表明，Tcf7l2通过激活NF-κB加速牙骨质形成，在牙周炎的治疗中具有巨大潜力，并为牙周组织再生提供指导。
Cementum regeneration is an important and challenging stage in periodontal tissue engineering and regeneration. Pathosis of the periodontium, including cementum, is important in precision diagnosis and obstinate treatment of systemic diseases, such as diabetes, leukemia, and Acquired Immune Deficiency Syndrome. Here, we found that during periodontium development, transcription factor 7‐like 2 (Tcf7l2) was widely expressed in the periodontium and dental sac. In mouse cementoblast cell line (OCCM‐30), the activation of NF‐κB and cementoblast mineralization was significantly reduced when Tcf7l2 gene was silenced. Moreover, Tcf7l2 has a positive effect on NF‐κB and cementoblast mineralization. Therefore, Tcf7l2 promotes cementum formation through the NF‐κB pathway. In addition, we found a decreased expression of phosphorylated p65 and a thin layer of cementum inTcf7l2fl/flmice. These results suggest that Tcf7l2, which accelerates cementum formation by activating NF‐κB, has great potential in the treatment of periodontitis and provide guidance for periodontal tissue regeneration.