Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA.
Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA.
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DOI：
10.3390/microorganisms6040110
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发表时间：
2018-10-20
期刊：
影响因子：
4.5
通讯作者：
Hirata A
中科院分区：
文献类型：
--
作者：
Hori H;Kawamura T;Awai T;Ochi A;Yamagami R;Tomikawa C;Hirata A
迄今为止，不仅在嗜热生物中，而且在嗜温生物中都已鉴定出 tRNA 中的许多修饰核苷以及 tRNA 修饰酶。由于嗜热菌 tRNA 中的大多数修饰核苷与嗜温菌 tRNA 中的核苷相同，因此它们被认为基本上在高温下蛋白质合成的步骤中起作用。在高温下，未修饰的 tRNA 结构会被破坏。因此，嗜热菌必须拥有稳定 tRNA 结构的策略。为此，已经鉴定了 tRNA 中的几种嗜热菌特异性修饰核苷。 RNA 结合蛋白和多胺等其他因素也有助于 tRNA 在高温下的稳定性。嗜热栖热菌是一种极端嗜热真细菌，它可以通过 tRNA 中的修饰核苷网络和 tRNA 修饰酶来调整其蛋白质合成系统以响应温度变化。值得注意的是，来自嗜热菌的 tRNA 修饰酶非常稳定。因此，它们已被用于生物化学和结构研究。将来，热稳定性 tRNA 修饰酶可能会用作生物技术工具，并可能用于医学科学。
To date, numerous modified nucleosides in tRNA as well as tRNA modification enzymes have been identified not only in thermophiles but also in mesophiles. Because most modified nucleosides in tRNA from thermophiles are common to those in tRNA from mesophiles, they are considered to work essentially in steps of protein synthesis at high temperatures. At high temperatures, the structure of unmodified tRNA will be disrupted. Therefore, thermophiles must possess strategies to stabilize tRNA structures. To this end, several thermophile-specific modified nucleosides in tRNA have been identified. Other factors such as RNA-binding proteins and polyamines contribute to the stability of tRNA at high temperatures. Thermus thermophilus, which is an extreme-thermophilic eubacterium, can adapt its protein synthesis system in response to temperature changes via the network of modified nucleosides in tRNA and tRNA modification enzymes. Notably, tRNA modification enzymes from thermophiles are very stable. Therefore, they have been utilized for biochemical and structural studies. In the future, thermostable tRNA modification enzymes may be useful as biotechnology tools and may be utilized for medical science.
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影响因子：
2.9
作者：
Mei, Xianghan;Alvarez, Jonathan;Bon Ramos, Adriana;Samanta, Uttamkumar;Iwata-Reuyl, Dirk;Swairjo, Manal A.
通讯作者：
Swairjo, Manal A.
影响因子：
16
作者：
Begley, Ulrike;Dyavaiah, Madhu;Begley, Thomas J.
通讯作者：
Begley, Thomas J.
影响因子：
4.8
作者：
Awai, Takako;Kimura, Satoshi;Hori, Hiroyuki
通讯作者：
Hori, Hiroyuki
DOI：
10.1073/pnas.0802247105
发表时间：
2008-05-13
影响因子：
11.1
作者：
Alian, Akram;Lee, Tom T.;Finer-Moore, Janet
通讯作者：
Finer-Moore, Janet
影响因子：
3.2
作者：
Black, Katherine A.;Dos Santos, Patricia C.
通讯作者：
Dos Santos, Patricia C.