Dysfunctional nurturing behavior in rat dams with limited access to nesting material: A clinically relevant model for early-life stress
Dysfunctional nurturing behavior in rat dams with limited access to nesting material: A clinically relevant model for early-life stress
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DOI：
10.1016/j.neuroscience.2008.04.019
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发表时间：
2008-06-26
期刊：
影响因子：
3.3
通讯作者：
Baram, T. Z.
中科院分区：
文献类型：
--
作者：
Ivy, A. S.;Brunson, K. L.;Baram, T. Z.
背景资料：早期生活中的情绪压力可能与以后生活中的情感和认知障碍有关，但用于研究潜在机制的令人满意的动物模型有限。由于母亲的存在和行为严重影响后代的分子和行为应激反应，我们试图建立一个模型的功能失调，支离破碎的母亲养育行为，这反过来又会引起慢性早期生活stressintheoffice.Methods：Sprague-Dawley大鼠母鼠的护理和养育行为的改变，限制他们的能力，创造满意的巢在产后2-9天。在整个昼夜周期中观察母性行为，并对养育行为的频率和持续时间进行评分。此外，潜在的压力和焦虑的母鼠进行了评估，使用行为，分子和激素measurements.Results：无论是数量和质量的母鼠照顾他们的小狗深刻的影响，限制筑巢材料在他们的笼子里：舔/梳理活动减少，离开小狗的频率增加，导致碎片之间的相互作用的母鼠和小狗。母鼠的异常活动模式伴随着开阔视野中焦虑样行为的增加，但在高架迷宫测试中则不然。此外，母鼠的血浆皮质酮水平和肾上腺重量增加，表明这些母鼠的慢性应激。结束的有限嵌套，应激诱导期，下丘脑促肾上腺皮质激素释放激素（CRH）的mRNA表达减少在有限嵌套的大坝，而精氨酸加压素（AVP）的mRNA水平没有显着affected.Conclusion：限制大坝的能力，建立一个巢，为他们的幼崽导致异常的剧目的养育行为，可能是由于慢性应激和轻度焦虑的大坝。由于分散和异常的母性行为引起了幼崽的慢性压力，有限的筑巢范式提供了一个有用的工具，研究这种早期生活压力的机制和后果的后代。(c)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Background: Early-life emotional stress may be associated with affective and cognitive disorders later in life, yet satisfactory animal models for studying the underlying mechanisms are limited. Because maternal presence and behavior critically influence molecular and behavioral stress responses in offspring, we sought to create a model of dysfunctional, fragmented maternal nurturing behavior that would, in turn, provoke chronic early-life stress in the offspring.Methods: Sprague-Dawley rat dams' nursing and nurturing behaviors were altered by limiting their ability to create satisfactory nests during postpartum days 2-9. Maternal behavior was observed throughout the diurnal cycle, and the frequency and duration of nurturing behaviors were scored. In addition, potential stress and anxiety of the dams were assessed using behavioral, molecular and hormonal measures.Results: Both the quantity and the quality of dams' care of their pups were profoundly influenced by restriction of nesting materials in their cages: licking/grooming activities decreased and the frequency of leaving the pups increased, resulting in fragmented interactions between the dams and pups. The abnormal activity patterns of the dams were accompanied by increased anxiety-like behavior in the open field, but not in the elevated plus maze tests. Additionally, dams' plasma corticosterone levels and adrenal weights were augmented, suggesting chronic stress of these dams. By the end of the limited-nesting, stress-inducing period, hypothalamic corticotropin releasing hormone (CRH) mRNA expression was reduced in the limited-nesting dams, while arginine-vasopressin (AVP) mRNA levels were not significantly affected.Conclusion: Limiting dams' ability to construct a nest for their pups leads to an abnormal repertoire of nurturing behaviors, possibly as a result of chronic stress and mild anxiety of the dams. Because the fragmented and aberrant maternal behavior provoked chronic stress in the pups, the limited-nesting paradigm provides a useful tool for studying the mechanisms and consequences of such early-life stress experience in the offspring. (c) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.