Propofol induces growth cone collapse and neurite retractions in chick explant culture
Propofol induces growth cone collapse and neurite retractions in chick explant culture
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DOI：
10.1007/bf03022874
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发表时间：
2006-11-01
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影响因子：
--
通讯作者：
Goto, Fumio
中科院分区：
文献类型：
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作者：
Al-Jahdari, Wael S.;Saito, Shigeru;Goto, Fumio
目的：异丙酚神经毒性已在几种细胞培养系统中得到证实。本研究旨在确定丙泊酚是否对外周神经元、视网膜神经元和自主神经元具有神经毒性作用，以及哪些神经元特别容易受到丙泊酚的损伤。从第8天的鸡胚中分离背根神经节、视网膜神经节细胞层和交感神经节链，并培养20小时。加入不同浓度[5-300 μ M（0.9-53 μ g.mL（-1））]的丙泊酚，以研究其对这三种类型的神经元组织的作用。形态学变化通过生长锥塌陷实验进行定量检测。使用高效液相色谱法测量异丙酚浓度。结果：异丙酚诱导生长锥塌陷和神经突破坏。在异丙酚作用后2 h，三种类型的神经元的量效关系有显著性差异（P < 0.001），但在24 h时无显著性差异。生长锥崩溃的影响是至少部分可逆的，在所有三种类型的神经元暴露后，以100 μ M丙泊酚长达6小时，虽然可逆性未观察到24小时expos.Conclusion：虽然丙泊酚的临床安全性已被证明，在高浓度丙泊酚在体外生长的神经元有潜在的神经毒性。
Purpose: Propofol neurotoxicity has been demonstrated in several cell culture systems. This study was undertaken to determine whether propofol has neurotoxic effects on peripheral, retinal, and autonomic neurons, and which neurons are particularly liable to injury by propofol.Method: Dorsal root ganglia, retinal ganglion cell layers, and sympathetic ganglion chains were isolated from day eight chick embryos and cultured for 20 hr. Thereafter, propofol was added at various concentrations [5-300 mu M (0.9-53 mu g.mL(-1))] to investigate its effects on these three types of neuronal tissue. Morphological changes were examined quantitatively by growth cone collapse assay. Propofol concentrations were measured using high performance liquid chromatography.Results: Propofol induced growth cone collapse and neurite destruction. The three types of neurons tested exhibited significantly different dose-response relationships two hours after the application of propofol (P < 0.001) but not at 24 hr after application. The growth cone-collapsing effect was at least partially reversible in all three types of neurons after exposure to 100 mu M propofol up to six hours, though reversibility was not observed after 24-hr exposure.Conclusion: While the clinical safety profile of propofol has been well documented, at high concentrations propofol has potential neurotoxicity on growing neurons in vitro.