Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus.
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus.
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DOI：
10.3389/fmicb.2022.868887
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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正痘病毒（OPXVs）不仅感染它们的自然宿主，而且一些OPXVs还可以在人类中引起疾病。以前，我们部分特点的OPXV隔离从一个18岁的男性生活在北方挪威。限制性内切酶分析和部分基因组测序鉴定该病毒为非典型牛痘病毒（CPXV），我们将其命名为CPXV-No-H2。在这项研究中，我们使用Illumina和Nanopore测序确定了CPXV-No-H2的完整基因组序列。结果表明，CPXV-No-H2全基因组长度为220，276 bp，末端反向重复序列长度约为7 kbp，包含217个预测基因。17个预测的CPXV-No-H2蛋白与来自旧世界的OPXV蛋白最相似，包括鼠痘病毒（ECTV）和牛痘病毒，以及北美的阿拉斯加痘病毒（AKPV）。CPXV-No-H2具有与其他OPXV基因最相似的基因的嵌合基因组，并且鉴定了七个潜在的重组事件。系统进化分析表明，CPXV-No-H2与CPXV德国分离株CPXV_GerMygEK938_17和CPXV_Ger2010_MKY形成了一个独立的进化枝，同源性分别为96.4%和96.3%，与ECTV-OPXV Abatino进化枝聚在一起。CPXV-No-H2是一种花叶病毒，可能是由OPXV之间的几次重组事件引起的，其系统发育聚类表明ECTV-Abatino样牛痘病毒形成了一个独特的牛痘病毒新分支。
Orthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which we named CPXV-No-H2. In this study, we determined the complete genome sequence of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World, including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus (AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV genes, and seven potential recombination events were identified. The phylogenetic analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3% nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several recombination events between OPXVs, and its phylogenetic clustering suggests that ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses.