Differential expression of S100 gene family in human esophageal squamous cell carcinoma
Differential expression of S100 gene family in human esophageal squamous cell carcinoma
复制标题
DOI：
10.1007/s00432-004-0555-x
复制
发表时间：
2004-08-01
影响因子：
3.6
通讯作者：
Liu, ZH
中科院分区：
文献类型：
--
作者：
Ji, JF;Zhao, LQ;Liu, ZH
目的。研究S100基因家族在人食管鳞状细胞癌（ESCC）中RNA水平的差异表达，探讨S100基因家族与ESCC的关系。方法。首先利用Software Primer 3针对不同的S100基因设计特异性引物，要求每个S100基因的两条引物序列分别来自两个不同的外显子。然后，通过半定量逆转录聚合酶链反应（RT-PCR）检测62例食管鳞癌与相应正常食管粘膜中16个S100基因的差异表达。所有RT-PCR产物均通过1.5%琼脂糖凝胶进行分析。借助Fluor-S MultiImager和Multi-Analyst软件，使用SAS 8.1软件对电泳图像进行统计分析。结果。 16 个 S100 基因中有 11 个显着下调 (p
Purpose. To study the differential expression of the S100 gene family at the RNA level in human esophageal squamous cell carcinoma (ESCC), and to find the relationship of the S100 gene family with ESCC. Methods. Firstly, the specific primers were designed for the different S100 genes with Software Primer 3, which required that both primer sequences of each S100 gene were from two different exons respectively. Then, the differential expression of 16 S100 genes was examined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in 62 cases of ESCC versus the corresponding normal esophageal mucosa. All RT-PCR products were analyzed by 1.5% agarose gel. With Fluor-S MultiImager and Multi-Analyst software, the electrophoresis images were evaluated with statistics analysis using SAS 8.1 software. Results. Eleven out of 16 S100 genes were significantly downregulated (p