Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species
Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species
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DOI：
10.1007/s001220100715
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发表时间：
2001-11-01
影响因子：
5.4
通讯作者：
Ogihara, Y
中科院分区：
文献类型：
--
作者：
Ishii, T;Mori, N;Ogihara, Y
从普通小麦中国春（TriticumaestivumcvChineseSpring）叶绿体DNA全序列中鉴定出24个含有10个以上重复序列的叶绿体微卫星位点。对每个微卫星序列设计一对引物，扩增出100-200 bp的特异性PCR产物。利用来自11个小麦属和山羊草属物种的43份材料，分析了微卫星位点的等位基因多样性。在24个叶绿体微卫星位点中，有21个位点出现多态性带型。这三个单态微卫星被发现位于编码区。多态性微卫星的等位基因数为2 ~ 7个，平均4.33个。多样性值（H）为0.05 ~ 0.72，平均值为0.47。显著相关（P
Twenty four chloroplast microsatellite loci having more than ten mononucleotide repeats were identified from the entire chloroplast DNA sequence of common wheat, Triticum aestivum cv Chinese Spring. For each microsatellite, a pair of primers were designed to produce specific PCR products in the range of 100-200 bp. The allelic diversity at the microsatellite loci was evaluated using 43 accessions from 11 Triticum and Aegilops species involved in wheat polyploid evolution. Polymorphic banding patterns were obtained at 21 out of 24 chloroplast microsatellite loci. The three monomorphic microsatellites were found to be located in coding regions. For the polymorphic microsatellites, the number of alleles per microsatellite ranged from 2 to 7 with an average of 4.33. and the diversity values (H) ranged from 0.05 to 0.72 with an average of 0.47. Significant correlations (P