PANNING FOR LYMPHOCYTES - METHOD FOR CELL SELECTION
PANNING FOR LYMPHOCYTES - METHOD FOR CELL SELECTION
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DOI：
10.1073/pnas.75.6.2844
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
SATO, VL
中科院分区：
文献类型：
--
作者：
WYSOCKI, LJ;SATO, VL
开发了一种分离T [胸腺衍生]和B [骨髓衍生]淋巴细胞的简单方法。涂有小鼠IG [免疫球蛋白]特异性抗体的塑料培养皿选择性结合脾B淋巴细胞。贴壁细胞很容易通过轻轻移液去除;贴壁和非贴壁群体保留免疫功能。在一个典型的实验中，当3 ×将107个脾淋巴细胞加入到100 × 104的培养皿中。用微克量的抗Ig包被15 mm塑料培养皿，98%的非贴壁细胞为Ig阴性，97%的贴壁细胞为Ig阳性。该方法是足够灵敏的，以允许检测和分离的细胞类型，包括少至2%的总人口，并可以修改，以允许选择细胞的双抗体程序。塑料培养皿法在根据细胞表面抗原分离细胞方面可能普遍有用。
A simple method for the fractionation of T [thymus-derived] and B [bone marrow-derived] lymphocytes was developed. Plastic dishes coated with antibodies specific for mouse Ig [immunoglobulin] selectively bound splenic B lymphocytes. The adherent cells were easily removed by gentle pipetting; adherent and nonadherent populations retained immunologic function. In a typical experiment, when 3 .times. 107 splenic lymphocytes were added to a 100 .times. 15 mm plastic dish coated with microgram quantities of anti-Ig, 98% of the nonadherent cells were Ig-negative and 97% of the adherent cells were Ig-positive. The method was sufficiently sensitive to allow detection and separation of cell types comprising as little as 2% of the total population and can be modified to allow the selection of cells by a double-antibody procedure. The plastic dish method will probably be generally useful for fractionating cells on the basis of their cell surface antigens.