DIFFERENTIAL GROWTH OF THE MOUSE PREIMPLANTATION EMBRYO IN CHEMICALLY-DEFINED MEDIA
DIFFERENTIAL GROWTH OF THE MOUSE PREIMPLANTATION EMBRYO IN CHEMICALLY-DEFINED MEDIA
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DOI：
10.1095/biolreprod50.5.1027
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发表时间：
1994-05-01
影响因子：
3.6
通讯作者：
BIGGERS, JD
中科院分区：
文献类型：
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作者：
ERBACH, GT;LAWITTS, JA;BIGGERS, JD
我们最近报道了使用顺序单纯形优化方法来设计一种培养基（SOM），该培养基克服了在小鼠远交品系的胚胎中体外发生的超过两个细胞的发育障碍。我们已经检查了这种培养基和其他几种培养基培养CF 1雌性X BGD 2F 1雄性小鼠胚胎发育通过胚泡阶段的能力。培养基SOM的改良，命名为KSOM，K+浓度增加（2.5 mM），也支持生长超过两细胞块;与测试的其他培养基相比，它产生更高的压实率（100%），提供更大的囊胚产量（88%），并刺激滋养层细胞的细胞分裂率增加。KSOM培养的胚泡的总细胞数（44 +/-12; n = 30）表明，当胚胎从原核合子培养36小时时，5-6次细胞分裂是可能的。这是一个显着的改善，性能超过其他确定的培养基的受精卵培养到囊胚。
We have recently reported the use of sequential simplex optimization methods to design a medium (SOM) that overcomes the block to development beyond two cells which occurs in vitro in embryos from an outbred strain of mouse. We have examined this medium and several others for their ability to foster development of CFl female x BGD2Fl male mouse embryos through the blastocyst stage. A modification of medium SOM, designated KSOM, with an increased concentration of K+ (2.5 mM), also supports growth beyond the two-cell block; compared to other media tested it produces a higher rate of compaction (100%), provides a larger yield of blastocysts (88%), and stimulates an increased rate of cell division of the trophoblast cells. The total cell number of KSOM-cultured blastocysts (44 +/- 12; n = 30) indicates that 5-6 cell divisions are possible when embryos are cultured for 36 h from pronuclear zygotes. This is a significant improvement in performance over that of other defined media for the culture of zygotes to blastocysts.