Uncoupling protein-2 messenger ribonucleic acid expression during very-low-calorie diet in obese premenopausal women
Uncoupling protein-2 messenger ribonucleic acid expression during very-low-calorie diet in obese premenopausal women
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DOI：
10.1210/jc.83.7.2450
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发表时间：
1998-07-01
影响因子：
5.8
通讯作者：
Langin, D
中科院分区：
文献类型：
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作者：
Barbe, P;Millet, L;Langin, D
解偶联蛋白-a (UCP2) 是一种在多种人体组织中表达的线粒体蛋白。通过将呼吸与 ATP 合成解偶联，UCP2 可能参与能量消耗的控制。我们研究了人类脂肪组织中 UCP2 基因的表达。在八名受试者中，我们发现皮下脂肪库和内脏脂肪库 UCP2 信使 RNA (mRNA) 水平之间呈正相关（r = 0.91，P < 0.002），这表明皮下脂肪组织中的 UCP2 mRNA 水平是全身脂肪组织中 UCP2 基因表达的良好指标。在 8 名肥胖绝经前女性中研究了 25 天极低热量饮食对 UCP2 mRNA 水平和静息代谢率的影响。饮食之前和期间UCP2 mRNA水平没有差异。低热量饮食 25 天后，发现脂肪组织 UCP2 mRNA 水平与根据去脂体重调整的静息代谢率呈正相关（r = 0.82，P < 0.01）。这些结果表明，与短期禁食不同，极低热量饮食与 UCP2 mRNA 表达的诱导无关，并且脂肪组织 UCP2 mRNA 水平可能与人类静息能量消耗的变化有关。
Uncoupling protein-a (UCP2) is a mitochondrial protein expressed in a wide range of human tissues. By uncoupling respiration from ATP synthesis, UCP2 might be involved in the control of energy expenditure. We have investigated UCP2 gene expression in human adipose tissue. In eight subjects, we found a positive correlation(r = 0.91, P < 0.002) between subcutaneous and visceral fat depots UCP2 messenger RNA (mRNA) levels, suggesting that UCP2 mRNA level in subcutaneous adipose tissue is a good index of UCP2 gene expression in whole body adipose tissues. The effect of a 25-day very-low-calorie diet on UCP2 mRNA level and resting metabolic rate was investigated in eight obese premenopausal women. There was no difference in UCP2 mRNA levels before and during the diet. After 25 days of hypocaloric diet, a positive correlation was found between adipose tissue UCP2 mRNA level and resting metabolic rate adjusted for lean body mass (r = 0.82, P < 0.01). These results show that very-low-calorie diet, unlike short-term fasting, is not associated with an induction in UCP2 mRNA expression, and that adipose tissue UCP2 mRNA levels may be related to variations in resting energy expenditure in humans.