Structure of a photoactive rhodium complex intercalated into DNA
Structure of a photoactive rhodium complex intercalated into DNA
复制标题
插入 DNA 的光活性铑配合物的结构
DOI：
10.1038/72385
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
D. Rees
中科院分区：
文献类型：
--
作者：
C. Kielkopf;K. E. Erkkila;B. Hudson;J. Barton;D. Rees
铑（III）的嵌入络合物是强光氧化剂，其促进DNA链切割或通过双螺旋的电子转移。与DNA螺旋结合的序列特异性铑嵌入剂的1.2 μ m分辨率晶体结构为铑嵌入剂的序列特异性提供了理论基础。它还解释了插入寡核苷酸中心如何改变DNA构象。铑配合物通过大沟插入，在大沟中与目标位点的碱基边缘形成特定接触。phi配体深深插入DNA碱基对堆栈中。DNA的主要构象变化是每个残基增加一倍，而B型DNA的糖折叠没有变化。基于在该结构中观察到的嵌入DNA螺旋的五个晶体学独立视图，嵌入剂可以被认为是位于大沟中的具有特定官能团的额外碱基对。
Intercalating complexes of rhodium(III) are strong photo-oxidants that promote DNA strand cleavage or electron transfer through the double helix. The 1.2 Å resolution crystal structure of a sequence-specific rhodium intercalator bound to a DNA helix provides a rationale for the sequence specificity of rhodium intercalators. It also explains how intercalation in the center of an oligonucleotide modifies DNA conformation. The rhodium complex intercalates via the major groove where specific contacts are formed with the edges of the bases at the target site. The phi ligand is deeply inserted into the DNA base pair stack. The primary conformational change of the DNA is a doubling of the rise per residue, with no change in sugar pucker from B-form DNA. Based upon the five crystallographically independent views of an intercalated DNA helix observed in this structure, the intercalator may be considered as an additional base pair with specific functional groups positioned in the major groove.
影响因子：
2.9
作者：
Jackson, BA;Alekseyev, VY;Barton, JK
通讯作者：
Barton, JK
影响因子：
--
作者：
C. Chow;J. Barton
通讯作者：
C. Chow;J. Barton
影响因子：
56.9
作者：
Kelley, SO;Barton, JK
通讯作者：
Barton, JK