Meat tenderness: Immunofluorescent localisation of the isomorphic forms of collagen in bovine muscles of varying texture
Meat tenderness: Immunofluorescent localisation of the isomorphic forms of collagen in bovine muscles of varying texture
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肉嫩度：不同质地牛肌肉中胶原蛋白同构形式的免疫荧光定位
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
V. Duance
中科院分区：
文献类型：
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作者：
A. Bailey;D. Restall;T. Sims;V. Duance
利用胶原型特异性抗体，通过间接免疫荧光法确定了肌肉中胶原蛋白的三种同构形式。I型胶原蛋白位于肌外膜和肌膜周围，III型胶原蛋白主要位于肌膜周围，呈细花边状纤维，V型胶原蛋白几乎局限于肌内膜。与半腱肌和背最长肌相比，在较硬的桡侧腕肌和胸直肌的胸膜周围，III型胶原蛋白的比例似乎有所增加。通过对溴化氰肽的分析证实了III型比例的增加。III型胶原比例增加与韧性增加的关系，如果有的话，仍有待阐明。
The three isomorphic forms of collagen present in muscle have been located by indirect immunofluorescence using collagen type specific antibodies. Type I collagen was located in the epimysium and perimysium, type III primarily in the perimysium as fine lace-like fibres, and type V virtually confined to the endomysium. Increased proportions of type III collagen appeared to be present in the perimysium of the tougher muscles M. carpi radialis, and M. sternomandibularis compared to M. semitendinosus and M. longissimus dorsi. This increased proportion of type III was confirmed by analysis of the cyanogen bromide peptides. The relationship of the increased proportion of type III collagen to increased toughness, if any, remains to be elucidated.